| Profilin-1 Inhibits Branched Polymerization of Actin Filaments by the Arp2/3 Complex and the VCA Domain of WASP. Actin polymerization was carried out as described in the method; all reactions contain a 1:1 ratio of pyrene- and nonlabeled-actin. Actin in the presence of both Arp2/3 and VCA shows enhancement of actin nucleation. Upon the addition of Profilin-1, the steep nucleation phase provided by both Arp2/3 and VCA is delayed and greatly reduced. The additionof Profilin-1 to actin also decreases the rate of actin polymerization when compared to actin alone. |
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