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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
见官方网站
- 亚型:
见官方网站
- 形态:
冻干粉或液体
- 保存条件:
-20ºC
- 克隆性:
多克隆
- 标记物:
见官方网站
- 适应物种:
见官方网站
- 保质期:
1年
- 抗原来源:
见官方网站
- 目录编号:
HYB 097-046
- 级别:
见官方网站
- 库存:
30
- 供应商:
上海泽叶生物科技有限公司
- 宿主:
Human
- 应用范围:
ELISA
- 浓度:
1 mg/ml
- 靶点:
见官方网站
- 抗体英文名:
Anti-α-fetoprotein (human, AFP)
- 抗体名:
Anti-α-fetoprotein (human, AFP)
- 规格:
200ug
Anti-α-fetoprotein (human, AFP)
Bioporto是一家体外诊断公司,专业为临床和科研提供一系列诊断试剂盒和抗体。其领先的产品组合包括了用于急性肾功能损伤诊断用的NGAL以及其它疾病检测用抗体。官方网站:www.bioporto.com
- Cat. No.
- HYB 097-046
- Product name
- Anti-α-fetoprotein (human, AFP)
- Description
-
Mouse monoclonal antibody
- Specificity
-
HYB 097-04 is specific for human AFP.
- Immunogen
-
Human α-fetoprotein isolated from amniotic fluid
- Application
- ELISA
- Species reactivity (positive)
- Human
- Species reactivity (negative)
- Not determined
- Subclass
-
IgG1/k
- Gene ID
- 174
- Clone number
- 10B8
- Epitope
-
Epitope belongs to group II
- Content
-
Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
- Purification
- Protein-A purified
- Form
- Liquid
- Solvent
- 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
- Storage
- 4-8ºC without exposure to light. No precautions necessary during handling.
- Target
-
α-fetoprotein (AFP) is a serum protein produced by hepatocytes. AFP levels are used as a tumor marker for hepatomas and germ cell tumors, and the serum concentration has prognostic significance in other diseases. Levels of AFP is 0.6-9.3 kIU/L in blood from normal individuals under the age of 40. Concentrations raise with age and during pregnancy.
AFP consists of one single polypeptide chain of 590 aminoacids with MW 65 kDa. - Application 1
-
ELISA
Ka=190, determined as concentration of antibody giving half-maximal binding to hepatoma-AFP bound to solid phase (unit: 108 L/mol). HYB 097-04 reacts strongly with human AFP. A strong reaction is seen when tested in sandwich ELISA in combination with a polyclonal antibody against human AFP (eg. DAKO A0008).
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文献和实验缩写 AFP.为仅在胎儿血清中存在的蛋白质(成人血清中没有),也称为胎球蛋白( fetuin)、α 1 -胎儿球蛋白(α 1 -fetoglobulin)、后清蛋白。在电泳实验中可在α -球蛋白区域(有的动物在β -球蛋白区域)找到。是在胎儿的肝脏中合成的。关于分子的性质,人的分子量为 6.46万, S20 , w=4.5S, D20 , w=6.18, pI为 pH=4.8,由单链多肽所成;而鼠的是由 586个氨基酸所成,其结构由三个( 1— 193, 194— 385
甲胎蛋白(α-fetoprotein,αFP或AFP)主要在胎儿肝中合成,分子量6.9万,在胎儿13周AFP占血浆蛋白总量的1/3。在妊娠30周达最高峰,以后逐渐下降,出生时血浆中浓度为高峰期的1%左右,约40mg/L,在周岁时接近成人水平(低于30μmg/L)。 甲胎蛋白在产妇羊水或母体血浆中AFP可用于胎儿产前监测。如在神经管缺损、脊柱裂、无脑儿等时,AFP可由开放的神经管进入羊水而导致其在羊水中含量显著升高。胎儿在宫腔内死亡、畸胎瘤等先天缺陷亦可有羊水中AFP
Immunofluorescence: Rabbit Anti-Murine RELMα
for 25 minutes. 3. Incubate the tissue section with blocking buffer for 20 minutes. 4. Incubate the tissue section overnight at 4?C with Rabbit Anti-Murine RELMα at 4.0 ng/mL in 1X PBS with 0.01% Triton-X and 0.5% BSA. Wash the slide
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