Double stranded DNA (dsDNA). Cross-reactions to single stranded DNA (ssDNA) and very weak reactivity with RNA on dot blots has been observed.
Immunogen
F1 hybrid from NZB Black and White mouse.
Application
IB, ELISA, IF
Species reactivity (positive)
Not determined
Species reactivity (negative)
Not determined
Subclass
IgG2a/k
Clone number
35 | 9 DNA
Epitope
Not determined
Content
Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
In combination with immunofluorescence the antibody is well suited for detection of dsDNA in fx. Crithidia luciliae, a monoflagellate protozoa, containing a giant mitochondrion (3).The antibody can be used for easy quantification of in vitro cell proliferation by ELISA and has successfully been used for monitoring cytotoxity and apoptosis as well as ELISA-based co-culture angiogenesis and proliferation assays (2).Very good reactivity both with dsDNA and ssDNA on NC-dotblots has been observed. The minimal size for DNA binding HYB 331-01 is >16 bases and there is an inverse proportionality between binding and ionic strength (1).
Application 1
Immunoblotting (IB) HYB 311-01 was used in IB (1).
Application 2
ELISA HYB 311-01 was used in ELISA (2).
Application 3
Immunofluorescence (IF) HYB 311-01 was used in IF (3).
Application 4
Immunogold staining (IGS) HYB 331-01 was used in IGS.
Immunogold staining (IGS) with HYB 331-01 and 15-nm gold particles. Reactivity inside nucleus and mitochondria of a human epithelial cancer cell is depicted (original magnification, 60,000x). Image courtesy of Marina Leite, Epithelial Interactions in Cancer (EPIC) group, i3s/IPATIMUP, Portugal. Pictures taken at the Histology and Electron Microscopy Service (HEMS) at i3s/IBMC by Rui Fernandes.