4-8ºC without exposure to light. No precautions necessary during handling.
克隆性:
多克隆
标记物:
见说明书
适应物种:
见说明书
保质期:
12个月
抗原来源:
Not determined
目录编号:
HYB 331-01
级别:
见说明书
库存:
大量
供应商:
上海信裕生物科技有限公司
宿主:
Not determined
应用范围:
IB, ELISA, IF
浓度:
见说明书
靶点:
见说明书
抗体英文名:
Anti-Doublestranded DNA
抗体名:
Anti-Doublestranded DNA
规格:
200ug/1mg
Anti-Doublestranded DNA
Cat. No.
HYB 331-01
Product name
Anti-Doublestranded DNA
Description
Mouse monoclonal antibody
Specificity
Double stranded DNA (dsDNA). Cross-reactions to single stranded DNA (ssDNA) and very weak reactivity with RNA on dot blots has been observed.
Immunogen
F1 hybrid from NZB Black and White mouse.
Application
IB, ELISA, IF
Species reactivity (positive)
Not determined
Species reactivity (negative)
Not determined
Subclass
IgG2a/k
Clone number
35 | 9 DNA
Epitope
Not determined
Content
Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
In combination with immunofluorescence the antibody is well suited for detection of dsDNA in fx. Crithidia luciliae, a monoflagellate protozoa, containing a giant mitochondrion (3).The antibody can be used for easy quantification of in vitro cell proliferation by ELISA and has successfully been used for monitoring cytotoxity and apoptosis as well as ELISA-based co-culture angiogenesis and proliferation assays (2).Very good reactivity both with dsDNA and ssDNA on NC-dotblots has been observed. The minimal size for DNA binding HYB 331-01 is >16 bases and there is an inverse proportionality between binding and ionic strength (1).