Anti-Albumin (human serum, HSA

Anti-Albumin (human serum, HSA)

Bioporto是一家体外诊断公司，专业为临床和科研提供一系列诊断试剂盒和抗体。其领先的产品组合包括了用于急性肾功能损伤诊断用的NGAL以及其它疾病检测用抗体。
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Cat. No.

HYB 192-01

Product name

Anti-Albumin (human serum, HSA)

Description

Mouse monoclonal antibody

Specificity

HYB 192-01 is specific for native and denatured human serum albumin (HSA).

Immunogen

Native HSA isolated from human plasma

Application

ELISA, WB

Species reactivity (positive)

Human

Species reactivity (negative)

Not determined

Subclass

IgG1/k

Gene ID

213

Clone number

5B11

Epitope

HYB 192-01 differs from 192-02 

Purification

Protein-A purified

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Target

Human serum albumin (HSA) consists of a single polypeptide chain of 584 amino acids, stabilized by 17  disulfide bridges. HSA is the most common protein in serum, it is produced in the liver and the concentration in serum is 35-50 mg/ml. HSA is often used as a carrier molecule because of its binding capacity, and thus mainly functions as the regulator of the colloidal osmotic pressure of the blood. The molecular weight is 67.5 kDa.

Application 1

ELISA
HYB 192-01 reacts strongly with native and denatured HSA. A strong reaction is seen in ELISA with native or denatured HSA directly coated onto the microtiter well, and also in sandwich ELISA in combination with a polyclonal antibody against HSA (e.g. DAKO A 001). 

Application 2

WB
In Western blotting after SDS-PAGE, HYB 192-01 reacts with native and denatured HSA in non-reduced form only.