Anti-Apolipoprotein B (human)

Anti-Apolipoprotein B (human)

Bioporto是一家体外诊断公司，专业为临床和科研提供一系列诊断试剂盒和抗体。其领先的产品组合包括了用于急性肾功能损伤诊断用的NGAL以及其它疾病检测用抗体。
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Cat. No.

HYB 069-02

Product name

Anti-Apolipoprotein B (human)

Description

Mouse monoclonal antibody

Specificity

HYB 069-02 is specific for apo B from human serum/plasma. No cross-reactivity is seen with human apolipoprotein A-1.

Immunogen

Human LDL 

Application

ELISA, WB

Species reactivity (positive)

Human

Species reactivity (negative)

Not determined

Subclass

IgG2b/k

Gene ID

338

Clone number

7B8

Epitope

Not determined

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification

Protein-A purified

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Target

Apolipoprotein B (apo B) in human plasma is a major protein of low density lipoproteins (LDL). The molecular mass is 260 kDa. Apo B binds to specific receptors on cell membranes and is involved in removal of LDL and very low density lipoprotein (VLDL) cholesterol from circulation

Application 1

ELISA
HYB 069-02 reacts with apo B from human serum/plasma, when used in sandwich ELISA in combination with a polyclonal antibody against apo B, and also with isolated LDL directly coated onto the microtiter plate. 

Application 2

WB
In Western blotting after SDS-PAGE HYB 069-02 binds to apo B in reduced as well as in non-reduced forms, using isolated LDL as antigen.

Application 3

IHC
HYB 062-02 has been used in IHC.

Formalin fixed paraffin-embedded human kidney tissue stained with HYB 069-02. Dilution: 1:400

Formalin fixed paraffin-embedded human tonsil tissue stained with HYB 069-02. Plasma of the veins were stained strongly. Dilution: 1:400

 

Application 4

IF
HYB 069-02 has been used in IF.

IF staining in human kidney tissue stained with HYB 069-02. Signal is strong in veins of glomerulus. Dilution: 1:100.