- ¥545
- BioPorto
- 丹麦
- HYB 069-02
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20ºC
- 保质期：
1年
- 库存：
30
- 供应商：
上海泽叶生物科技有限公司
- 抗体英文名：
Anti-Apolipoprotein B (human)
- 规格：
200ug
Anti-Apolipoprotein B (human)
Bioporto是一家体外诊断公司，专业为临床和科研提供一系列诊断试剂盒和抗体。其领先的产品组合包括了用于急性肾功能损伤诊断用的NGAL以及其它疾病检测用抗体。官方网站：www.bioporto.com
- Cat. No.
- HYB 069-02
- Product name
- Anti-Apolipoprotein B (human)
- Description
-
Mouse monoclonal antibody
- Specificity
-
HYB 069-02 is specific for apo B from human serum/plasma. No cross-reactivity is seen with human apolipoprotein A-1.
- Immunogen
-
Human LDL
- Application
- ELISA, WB
- Species reactivity (positive)
- Human
- Species reactivity (negative)
- Not determined
- Subclass
-
IgG2b/k
- Gene ID
- 338
- Clone number
- 7B8
- Epitope
-
Not determined
- Content
-
Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
- Purification
- Protein-A purified
- Form
- Liquid
- Solvent
- 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
- Storage
- 4-8ºC without exposure to light. No precautions necessary during handling.
- Target
-
Apolipoprotein B (apo B) in human plasma is a major protein of low density lipoproteins (LDL). The molecular mass is 260 kDa. Apo B binds to specific receptors on cell membranes and is involved in removal of LDL and very low density lipoprotein (VLDL) cholesterol from circulation
- Application 1
-
ELISA
HYB 069-02 reacts with apo B from human serum/plasma, when used in sandwich ELISA in combination with a polyclonal antibody against apo B, and also with isolated LDL directly coated onto the microtiter plate. - Application 2
-
WB
In Western blotting after SDS-PAGE HYB 069-02 binds to apo B in reduced as well as in non-reduced forms, using isolated LDL as antigen. - Application 3
-
IHC
HYB 062-02 has been used in IHC.
Formalin fixed paraffin-embedded human kidney tissue stained with HYB 069-02. Dilution: 1:400 
Formalin fixed paraffin-embedded human tonsil tissue stained with HYB 069-02. Plasma of the veins were stained strongly. Dilution: 1:400 - Application 4
-
IF
HYB 069-02 has been used in IF.
IF staining in human kidney tissue stained with HYB 069-02. Signal is strong in veins of glomerulus. Dilution: 1:100.
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文献和实验Purification of Rat Hepatic Lipase Essentially Free of Apolipoprotein E and Apolipoprotein B
subfractions. In isolated, perfused rat livers inhibition of hepatic lipase with antibodies or removal of HL by heparin perfusion reduces the uptake of chylomicron remnants (13 ). Finally, recent studies with cultured hepatoma cells over-expressing human HL
人的载脂蛋白有两种表达形式: apoB-48和apoB-100。该基因的序列在肝、肠组织细胞中是相同的,但产物不同。在肝细胞中,产物为4563个氨基酸残基的肽：apoB-100; 但在肠细胞中,产物为只含2152个氨基酸残基的apoB-48, 缺失了apoB-100的N端同LDL受体结合的结构域。原因是在mRNA水平上将2153位的谷氨酰胺的密码子CAA中的C编辑成为U,形成一个终止密码子�UAA,结果使蛋白质的合成提前终止,合成一个新的相对分子质量约为250kDa的蛋白
a simple ELISA method to detect human anti-antibody response.
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