Anti-Peptide YY (human, PYY)

Anti-Peptide YY (human, PYY)

Bioporto是一家体外诊断公司，专业为临床和科研提供一系列诊断试剂盒和抗体。其领先的产品组合包括了用于急性肾功能损伤诊断用的NGAL以及其它疾病检测用抗体。
官方网站：www.bioporto.com

Cat. No.

ABS 029-01

Product name

Anti-Peptide YY (human, PYY)

Description

Mouse monoclonal antibody

Specificity

ABS 029-01 binds human PYY. Cross-reactivity with NPY or PP in solution is <0.1%.

Immunogen

Synthetic human PYY

Application

ELISA, IHC

Species reactivity (positive)

Human

Species reactivity (negative)

Not determined

Subclass

IgG1/k

Gene ID

5697

Clone number

1

Epitope

Not determined

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification

Protein-A purified

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Target

Peptide tyrosine-tyrosine (PYY) amide is a 36 amino acid. PYY is a member of the pancreatic polypeptide (PP) family of peptides (neuropeptide Y (NPY), PYY and PP). While NPY is localized in neurons (both in the central and peripheral nervous system) and PP in the pancreatic islets, PYY is found in both neurons and the gut. PYY is released in response to food intake from the same endocrine cells (L cells) in the intestinal mucosa as the glucagon-like peptides and inhibits gall bladder secretion, gut motility, and pancreatic secretion. These effects are similar to those of PP and overlap with the gut inhibitory activity of GLP-1

Application 1

ELISA
ABS 029-01 binds free PYY in solution. 
ABS 029-01 reacts in ELISA with PYY coated directly onto the microtiter well. Biotinylated ABS 029-01 can be used as detection antibody with ABS 051-04 as capture antibody for measuring PYY(3-36) by sandwich ELISA.
 

The calibration curve of a sandwich assay for PYY using ABS 051-04 as the capture antibody and ABS 029-01B as the biotinylated detection antibody.

Application 2

IHC
ABS 029-01 can be used in IHC. Please consult www.proteinatlas.org
 

Paraffin-embedded human small intestine tissue immunostained with ABS 029-01 (www.proteinatlas.org)

Paraffin-embedded human small intestine tissue immunostained with ABS 029-01 (www.proteinatlas.org)