Anti-Neuropeptide Y (human, ra

Anti-Neuropeptide Y (human, rat, mouse)

Bioporto是一家体外诊断公司，专业为临床和科研提供一系列诊断试剂盒和抗体。其领先的产品组合包括了用于急性肾功能损伤诊断用的NGAL以及其它疾病检测用抗体。
官方网站：www.bioporto.com

Cat. No.

ABS 028-08

Product name

Anti-Neuropeptide Y (human, rat, mouse)

Description

Mouse monoclonal antibody

Specificity

ABS 028-08 binds full length human/rat Neuropeptide Y (NPY). Cross-reactivity with NPY (1-24) , NPY (18-36), PYY or PP was <0.2%.

Immunogen

Synthetic human/rat neuropeptide Y 

Application

ELISA, IHC-P, IF

Species reactivity (positive)

Human, rat, mouse

Species reactivity (negative)

Not determined

Subclass

IgG1/k

Gene ID

4852, 24604

Clone number

8

Epitope

The epitope of ABS 028-08 is probably around the mid-molecular region, NPY (18-24).

Content

Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification

Protein-A purified

Form

Liquid

Solvent

0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide

Storage

4-8ºC without exposure to light. No precautions necessary during handling.

Target

Neuropeptide Y (NPY) is a 36 amino acid residue peptide with sequence and 3-D structural homology with PYY and pancreatic polypeptide (PP). NPY serves as a neurotransmitter and is exclusively localized in neurons in the central and peripheral nervous system. NPY is associated with the regulation of heart rate and food intake in several mammalian species.

Application 1

ELISA
ABS 028-08 binds free NPY in solution and ABS 028-08 reacts in ELISA directly with NPY coated directly onto the microtiter well.

Application 2

IHC
ABS 028-08 works well in IHC on paraffin embedded tissue sections.
 

Paraffin embedded mouse brain tissue (fibres in the hilus of the hippocampus) from a mouse suffering an epiletic attack just before perfusion stained with ABS 028-08 diluted 1/1000

Enlarged section of a paraffin embedded mouse brain tissue (fibres in the hilus of the hippocampus) from a mouse suffering an epileptic attack just before perfusion stained with ABS 028-08 diluted 1/1000

Paraffin embedded mouse brain tissue (interneurons in the cortex) stained with ABS 028-08 diluted 1/1000

Application 3

IF
ABS 028-08 works well in IF.

ABS 028-08 tested on tissue from human cerebral cortex, applying ultra thin sections using a technique called array tomography. (antibody: green, DAPI-stained nuclei: blue). The antibody labels NPY-positive processes in the neutrophil. Courtesy of Dr. Nancy O'Rourke, Stanford University.

 

ABS 028-08 tested on tissue from human cerebral cortex, applying ultra thin sections using a technique called array tomography. (antibody: green, DAPI-stained nuclei: blue). The antibody labels NPY-positive processes in the neutrophil. Courtesy of Dr. Nancy O'Rourke, Stanford University.