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- 供应商:
江蓝纯
- 规格:
20次
| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| D7010S | Seamless Cloning Kit (无缝克隆试剂盒) | 20次 | 298.00元 |
Seamless Cloning Kit(无缝克隆试剂盒)是一种新型的基于插入片段和线性化载体的末端进行同源重组的基因克隆试剂盒。本试剂盒利用包含了DNA 5' 外切酶(5' Exonuclease)、DNA聚合酶(DNA Polymerase)和DNA连接酶(DNA
Ligase)活性的重组酶(assembly enzyme),通过同源重组的方法可以将一个或多个DNA片段按照预定方向、快速、高效和精确
地插入到线性化载体中,并且终构建的克隆没有任何额外的碱基序列,因此被称作“无缝克隆”。
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文献和实验Seamless Ligation Cloning Extract (SLiCE) Cloning Method
. SLiCE overcomes the sequence limitations of traditional cloning methods, facilitates seamless cloning by recombining short end homologies (15–52 bp) with or without flanking heterologous sequences and provides an effective strategy for directional
BioBrick Assembly Using the In-Fusion PCR Cloning Kit
™ assembly normally involves assembly of two BioBricks™ at a time using restriction enzymes and DNA ligase. Here we describe an alternative BioBrick™ assembly protocol that describes the assembly of two BioBricks™ using the In-Fusion PCR Cloning Kit
Nature of the InsertThe cloning of PCR-amplified fragments into a linear vector is typically a rapid and efficient process. However, not all PCR fragments will clone with the same efficiency into the same vector. These differences
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