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- MedChemExpress(MCE)
- 中国
- HY-K1041-50 rxns
- 2025年07月14日
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- 供应商：
广州威佳科技有限公司
- 规格：
50 rxns
MCE Seamless DNA Assembly Plus Kit 无缝克隆试剂盒为新一代重组克隆试剂盒，一次反应可完成单个至多个 DNA 片段的重组。其中经优化的 Mix 预混了重组酶和反应缓冲液，并添加了辅助因子，可显著提高克隆的重组效率和对杂质的耐受度，最快仅需 5 分钟即可完成单片段重组，且阳性率高达 95% 以上。
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文献和实验Overview This is a method to assemble two BioBricks using the Clontech In-Fusion PCR Cloning Kit and maintains BioBrick standard formats. There are currently several BioBrick assembly standardsthat all involve assembly by restriction enzyme
BioBrick Assembly Using the In-Fusion PCR Cloning Kit
™ assembly normally involves assembly of two BioBricks™ at a time using restriction enzymes and DNA ligase. Here we describe an alternative BioBrick™ assembly protocol that describes the assembly of two BioBricks™ using the In-Fusion PCR Cloning Kit
Recombination-Based DNA Assembly and Mutagenesis Methods for Metabolic Engineering
In recent years there has been a growing interest in the precise and concerted assembly of multiple DNA fragments of diverse sizes, including chromosomes, and the fine tuning of gene expression levels and protein activity. Commercial DNA
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