大家都在搜
手机验证
-20℃
6个月
pGL4.73[hRluc/SV40]
大量
钰博生物
1ug/5ug
出品公司: | Ybscience |
---|---|
载体名称: | pGL4.73 ; pGL4.73[hRluc/SV40] |
质粒类型: | 信号通路报告载体;哺乳动物载体;海肾荧光素酶报告载体 |
高拷贝/低拷贝: | -- |
克隆方法: | 限制性内切酶,多克隆位点 |
启动子: | SV40 |
载体大小: | 3921 bp |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | -- |
载体标签: | hRluc |
载体抗性: | 氨苄青霉素 |
筛选标记: | 无 |
克隆菌株: | TOP10等常规菌株 |
宿主细胞(系): | HEK293等 |
备注: | pGL4.73[luc2P]载体是信号通路报告载体;含海肾 荧光素酶报告基因hRluc。 |
稳定性: | 瞬表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 非病毒 |
pGL4.73载体简介The pGL4.73[hRluc/SV40] Vector encodes the luciferase reporter gene hRluc (Renilla reniformis) and is designed for high expression and reduced anomalous transcription. The pGL4 Vectors are engineered with fewer consensus regulatory sequences and a synthetic gene, which has been codon optimized for mammalian expression.The pGL4.73[hRluc/SV40] Vector contains the hRluc reporter gene and an SV40 early enhancer/promoter and can be used as an expression control or a co-reporter vector.
LOCUS AY738229 3921 bp DNA circular SYN 08-NOV-2004DEFINITION Renilla luciferase reporter vector pGL4.73[hRluc/SV40], complete sequence.ACCESSION AY738229VERSION AY738229.1 GI:55535639KEYWORDS .SOURCE Renilla luciferase reporter vector pGL4.73[hRluc/SV40] ORGANISM Renilla luciferase reporter vector pGL4.73[hRluc/SV40] other sequences; artificial sequences; vectors.REFERENCE 1 (bases 1 to 3921) AUTHORS Almond,B., Chauvin,F. and Browning,J. TITLE pGL4 Luciferase Reporter Vectors Technical Manual, TM259, Promega Corporation JOURNAL UnpublishedREFERENCE 2 (bases 1 to 3921) AUTHORS Almond,B., Chauvin,F. and Browning,J. TITLE Direct Submission JOURNAL Submitted (31-AUG-2004) Scientific Communications, Promega Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USAFEATURES Location/Qualifiers source 1..3921 /organism="Renilla luciferase reporter vector pGL4.73[hRluc/SV40]" /mol_type="other DNA" /db_xref="taxon:296666" regulatory 51..469 /regulatory_class="promoter" /note="SV40 early" CDS 499..1434 /codon_start=1 /transl_table=11 /product="luciferase hRluc" /protein_id="AAV52883.1" /db_xref="GI:55535640" /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAE NAVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLT AWFELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIE EDIALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLS WPREIPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNT EFVKVKGLHFSQEDAPDEMGKYIKSFVERVLKNEQ" misc_feature 1466..1687 /note="SV40 late poly(A) region" primer_bind 1755..1774 /note="reporter vector primer 4 (RVPrimer4)" rep_origin 2012 /note="ColE1-derived" gene complement(2803..3663) /gene="ampR" CDS complement(2803..3663) /gene="ampR" /note="AmpR" /codon_start=1 /transl_table=11 /product="synthetic beta-lactamase" /protein_id="AAV52884.1" /db_xref="GI:55535641" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" regulatory 3768..3921 /regulatory_class="polyA_signal_sequence" /note="synthetic; transcriptional pause site" primer_bind complement(3870..3889) /note="reporter vector primer 3 (RVPrimer3)"ORIGIN 1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcagatct gcgcagcacc 61 atggcctgaa ataacctctg aaagaggaac ttggttaggt accttctgag gcggaaagaa 121 ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag 181 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc 241 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc 301 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg 361 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca 421 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttg gcaatccggt 481 actgttggta aagccaccat ggcttccaag gtgtacgacc ccgagcaacg caaacgcatg 541 atcactgggc ctcagtggtg ggctcgctgc aagcaaatga acgtgctgga ctccttcatc 601 aactactatg attccgagaa gcacgccgag aacgccgtga tttttctgca tggtaacgct 661 gcctccagct acctgtggag gcacgtcgtg cctcacatcg agcccgtggc tagatgcatc 721 atccctgatc tgatcggaat gggtaagtcc ggcaagagcg ggaatggctc atatcgcctc 781 ctggatcact acaagtacct caccgcttgg ttcgagctgc tgaaccttcc aaagaaaatc 841 atctttgtgg gccacgactg gggggcttgt ctggcctttc actactccta cgagcaccaa 901 gacaagatca aggccatcgt ccatgctgag agtgtcgtgg acgtgatcga gtcctgggac 961 gagtggcctg acatcgagga ggatatcgcc ctgatcaaga gcgaagaggg cgagaaaatg 1021 gtgcttgaga ataacttctt cgtcgagacc atgctcccaa gcaagatcat gcggaaactg 1081 gagcctgagg agttcgctgc ctacctggag ccattcaagg agaagggcga ggttagacgg 1141 cctaccctct cctggcctcg cgagatccct ctcgttaagg gaggcaagcc cgacgtcgtc 1201 cagattgtcc gcaactacaa cgcctacctt cgggccagcg acgatctgcc taagatgttc 1261 atcgagtccg accctgggtt cttttccaac gctattgtcg agggagctaa gaagttccct 1321 aacaccgagt tcgtgaaggt gaagggcctc cacttcagcc aggaggacgc tccagatgaa 1381 atgggtaagt acatcaagag cttcgtggag cgcgtgctga agaacgagca gtaattctag 1441 agtcggggcg gccggccgct tcgagcagac atgataagat acattgatga gtttggacaa 1501 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct 1561 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt 1621 atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa 1681 tgtggtaaaa tcgataagga tccgtcgacc gatgcccttg agagccttca acccagtcag 1741 ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat 1801 catgcaactc gtaggacagg tgccggcagc gctcttccgc ttcctcgctc actgactcgc 1861 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 1921 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 1981 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 2041 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 2101 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 2161 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 2221 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 2281 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 2341 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 2401 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag 2461 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 2521 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 2581 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 2641 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 2701 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 2761 cttggtctga cagcggccgc aaatgctaaa ccactgcagt ggttaccagt gcttgatcag 2821 tgaggcaccg atctcagcga tctgcctatt tcgttcgtcc atagtggcct gactccccgt 2881 cgtgtagatc actacgattc gtgagggctt accatcaggc cccagcgcag caatgatgcc 2941 gcgagagccg cgttcaccgg cccccgattt gtcagcaatg aaccagccag cagggagggc 3001 cgagcgaaga agtggtcctg ctactttgtc cgcctccatc cagtctatga gctgctgtcg 3061 tgatgctaga gtaagaagtt cgccagtgag tagtttccga agagttgtgg ccattgctac 3121 tggcatcgtg gtatcacgct cgtcgttcgg tatggcttcg ttcaactctg gttcccagcg 3181 gtcaagccgg gtcacatgat cacccatatt atgaagaaat gcagtcagct ccttagggcc 3241 tccgatcgtt gtcagaagta agttggccgc ggtgttgtcg ctcatggtaa tggcagcact 3301 acacaattct cttaccgtca tgccatccgt aagatgcttt tccgtgaccg gcgagtactc 3361 aaccaagtcg ttttgtgagt agtgtatacg gcgaccaagc tgctcttgcc cggcgtctat 3421 acgggacaac accgcgccac atagcagtac tttgaaagtg ctcatcatcg ggaatcgttc 3481 ttcggggcgg aaagactcaa ggatcttgcc gctattgaga tccagttcga tatagcccac 3541 tcttgcaccc agttgatctt cagcatcttt tactttcacc agcgtttcgg ggtgtgcaaa 3601 aacaggcaag caaaatgccg caaagaaggg aatgagtgcg acacgaaaat gttggatgct 3661 catactcgtc ctttttcaat attattgaag catttatcag ggttactagt acgtctctca 3721 aggataagta agtaatatta aggtacggga ggtattggac aggccgcaat aaaatatctt 3781 tattttcatt acatctgtgt gttggttttt tgtgtgaatc gatagtacta acatacgctc 3841 tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc agtgcaagtg 3901 caggtgccag aacatttctc t
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。