pACYCDuet-1

pACYCDuet-1

pACYCDuet1载体基本信息

出品公司:	Ybscience
别名:	pACYCDuet1, pACYCDuet 1
质粒类型:	大肠杆菌蛋白双基因表达载体，有两个多克隆位点
表达水平:	高
克隆方法:	多克隆位点，限制性内切酶
载体大小:	4008 bp
5' 测序引物:	T7
5' 测序引物序列:	T7: 5'-TAATACGACTCACTATAGGG-3';
3' 测序引物:	T7t
3' 测序引物序列:	T7t: 5'-TGCTAGTTATTGCTCAGCGG-3'
载体标签:	N-His, C-S
载体抗性:	氯霉素
备注:	pACYCDuet-1 is designed for the coexpression of two target genes. pACYCDuet-1 carries the P15A replicon, lacI gene and chloramphenicol resistance gene.
稳定性:	瞬时表达 Transient
组成型:	组成型 Constitutive
病毒/非病毒:	非病毒

pACYCDuet1载体质粒图谱和多克隆位点信息

pACYCDuet1载体简介

pACYCDue-1 is designed for the coexpression of two target genes. The vector contains two multiple cloning sites (MCS), each of which is preceded by a T7 promoter/lac operator and ribosome binding site (rbs). The vector also carries the P15A replicon, lacI gene and chloramphenicol resistance gene. This vector can be used in combination with pACYCDuet™-1 (Cat. No. 71146-3) in an appropriate host strain for the coexpression of up to 4 target genes. Genes inserted into MCS1 can be sequenced using the ACYCDuetUP1 Primer (Cat. No. 71178-3) and DuetDOWN1 Primer (Cat. No. 71179- 3). Genes inserted into MCS2 can be sequenced using the DuetUP2 Primer (Cat. No. 71180-3) and T7 Terminator Primer (Cat. No. 69337-3).

pACYCDuet1载体序列

LOCUS       pACYCDuet-1    4008 bp     DNA  Circular  SYN
DEFINITION  pACYCDuet-1
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTOAUTHORAD1|Novagen Inc.|
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="pACYCDuet-1"
                     /mol_type="other DNA"
     misc_feature    2..29
                     /label="lacO"
     promoter        214..232
                     /label="T7_promoter"
     misc_feature    232..259
                     /label="lacO"
     misc_feature    complement(448..466)
                     /label="T7_Terminal_primer"
     gene            complement(729..1388)
                     /label="CAT/CamR (variant)"
                     /gene="CAT/CamR (variant)"
     CDS             complement(729..1388)
                     /label="ORF frame 2"
     rep_origin      1750..2661
                     /label="p15A_origin"       
     misc_feature    complement(2786..3877)
                     /label="lacI"           
     CDS             complement(2786..3745)
                     /label="ORF frame 3"            
     CDS             complement(3618..158)
                     /label="ORF frame 2"            
     promoter        3992..2
                     /label="T7_promoter"                  
ORIGIN
    1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag
   61 gagatatacc atgggcagca gccatcacca tcatcaccac agccaggatc cgaattcgag
  121 ctcggcgcgc ctgcaggtcg acaagcttgc ggccgcataa tgcttaagtc gaacagaaag
  181 taatcgtatt gtacacggcc gcataatcga aattaatacg actcactata ggggaattgt
  241 gagcggataa caattcccca tcttagtata ttagttaagt ataagaagga gatatacata
  301 tggcagatct caattggata tcggccggcc acgcgatcgc tgacgtcggt accctcgagt
  361 ctggtaaaga aaccgctgct gcgaaatttg aacgccagca catggactcg tctactagcg
  421 cagcttaatt aacctaggct gctgccaccg ctgagcaata actagcataa ccccttgggg
  481 cctctaaacg ggtcttgagg ggttttttgc tgaaacctca ggcatttgag aagcacacgg
  541 tcacactgct tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt
  601 aacgaccctg ccctgaaccg acgaccgggt cgaatttgct ttcgaatttc tgccattcat
  661 ccgcttatta tcacttattc aggcgtagca ccaggcgttt aagggcacca ataactgcct
  721 taaaaaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt
  781 ctgccgacat ggaagccatc acagacggca tgatgaacct gaatcgccag cggcatcagc
  841 accttgtcgc cttgcgtata atatttgccc atagtgaaaa cgggggcgaa gaagttgtcc
  901 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa
  961 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
 1021 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
 1081 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
 1141 accagctcac cgtctttcat tgccatacgg aactccggat gagcattcat caggcgggca
 1201 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
 1261 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc
 1321 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
 1381 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
 1441 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat
 1501 tttcgccaaa agttggccca gggcttcccg gtatcaacag ggacaccagg atttatttat
 1561 tctgcgaagt gatcttccgt cacaggtatt tattcggcgc aaagtgcgtc gggtgatgct
 1621 gccaacttac tgatttagtg tatgatggtg tttttgaggt gctccagtgg cttctgtttc
 1681 tatcagctgt ccctcctgtt cagctactga cggggtggtg cgtaacggca aaagcaccgc
 1741 cggacatcag cgctagcgga gtgtatactg gcttactatg ttggcactga tgagggtgtc
 1801 agtgaagtgc ttcatgtggc aggagaaaaa aggctgcacc ggtgcgtcag cagaatatgt
 1861 gatacaggat atattccgct tcctcgctca ctgactcgct acgctcggtc gttcgactgc
 1921 ggcgagcgga aatggcttac gaacggggcg gagatttcct ggaagatgcc aggaagatac
 1981 ttaacaggga agtgagaggg ccgcggcaaa gccgtttttc cataggctcc gcccccctga
 2041 caagcatcac gaaatctgac gctcaaatca gtggtggcga aacccgacag gactataaag
 2101 ataccaggcg tttcccctgg cggctccctc gtgcgctctc ctgttcctgc ctttcggttt
 2161 accggtgtca ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga cactcagttc
 2221 cgggtaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg
 2281 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gaaagacatg caaaagcacc
 2341 actggcagca gccactggta attgatttag aggagttagt cttgaagtca tgcgccggtt
 2401 aaggctaaac tgaaaggaca agttttggtg actgcgctcc tccaagccag ttacctcggt
 2461 tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt
 2521 tttcagagca agagattacg cgcagaccaa aacgatctca agaagatcat cttattaatc
 2581 agataaaata tttctagatt tcagtgcaat ttatctcttc aaatgtagca cctgaagtca
 2641 gccccatacg atataagttg taattctcat gttagtcatg ccccgcgccc accggaagga
 2701 gctgactggg ttgaaggctc tcaagggcat cggtcgagat cccggtgcct aatgagtgag
 2761 ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
 2821 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca
 2881 gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc accgcctggc
 2941 cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga aaatcctgtt
 3001 tgatggtggt taacggcggg atataacatg agctgtcttc ggtatcgtcg tatcccacta
 3061 ccgagatgtc cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg
 3121 ccatctgatc gttggcaacc agcatcgcag tgggaacgat gccctcattc agcatttgca
 3181 tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct atcggctgaa
 3241 tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc gagacagaac
 3301 ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc
 3361 ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc tggtcagaga
 3421 catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg gcatcctggt
 3481 catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg
 3541 ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg ctggcaccca
 3601 gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc agggccagac
 3661 tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt gccacgcggt
 3721 tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa
 3781 cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg gcatactctg
 3841 cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc tcttccgggc
 3901 gctatcatgc cataccgcga aaggttttgc gccattcgat ggtgtccggg atctcgacgc
 3961 tctcccttat gcgactcctg cattaggaaa ttaatacgac tcactata