pKD3

pKD3

载体基本信息

出品公司:	Ybscience
载体名称:	pKD3
质粒类型:	细菌表达；基因敲除系统
高拷贝/低拷贝:	--
启动子:	R6K
克隆方法:	多克隆位点，限制性内切酶
载体大小:	2804 bp
5' 测序引物及序列:	R6K-3
3' 测序引物及序列:	rrnB-T1-term-F
载体标签:	--
载体抗性:	Ampicillin 和 Chloramphenicol
筛选标记:	--
备注:	--
稳定性:	--
组成型:	--
病毒/非病毒:	--

 

载体质粒图谱和多克隆位点信息

 

载体简介

 

载体序列

LOCUS       pKD3	2804 bp 	DNA	SYN
DEFINITION  pKD3
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="pKD3"
                     /mol_type="other DNA"
     misc_feature    complement(51..98)
                     /label="FRT"
     CDS             complement(134..772)
                     /label="ORF frame 2"
     misc_feature    1419..1437
                     /label="pUni_fwd_primer"
     gene            complement(1516..2376)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(1516..2376)
                     /label="ORF frame 3"
     promoter        complement(2418..2446)
                     /label="AmpR_promoter"
     CDS             complement(2452..100)
                     /label="ORF frame 2"
     terminator      2575..2618
                     /label="rrnB_T1_terminator"
     terminator      2750..2777
                     /label="rrnB_T2_terminator"
ORIGIN
    1 agattgcagc attacacgtc ttgagcgatt gtgtaggctg gagctgcttc gaagttccta
   61 tactttctag agaataggaa cttcggaata ggaacttcat ttaaatggcg cgccttacgc
  121 cccgccctgc cactcatcgc agtactgttg tattcattaa gcatctgccg acatggaagc
  181 catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg
  241 tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta
  301 aatcaaaact ggtgaaactc acccagggat tggctgagac gaaaaacata ttctcaataa
  361 accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt
  421 gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt
  481 gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt
  541 tcattgccat acgtaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg
  601 ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct
  661 gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac
  721 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt
  781 ccttagctcc tgaaaatctc gacaactcaa aaaatacgcc cggtagtgat cttatttcat
  841 tatggtgaaa gttggaacct cttacgtgcc gatcaacgtc tcattttcgc caaaagttgg
  901 cccagggctt cccggtatca acagggacac caggatttat ttattctgcg aagtgatctt
  961 ccgtcacagg taggcgcgcc gaagttccta tactttctag agaataggaa cttcggaata
 1021 ggaactaagg aggatattca tatggaccat ggctaattcc catgtcagcc gttaagtgtt
 1081 cctgtgtcac tgaaaattgc tttgagaggc tctaagggct tctcagtgcg ttacatccct
 1141 ggcttgttgt ccacaaccgt taaaccttaa aagctttaaa agccttatat attctttttt
 1201 ttcttataaa acttaaaacc ttagaggcta tttaagttgc tgatttatat taattttatt
 1261 gttcaaacat gagagcttag tacgtgaaac atgagagctt agtacgttag ccatgagagc
 1321 ttagtacgtt agccatgagg gtttagttcg ttaaacatga gagcttagta cgttaaacat
 1381 gagagcttag tacgtgaaac atgagagctt agtacgtact atcaacaggt tgaactgcgg
 1441 atcttgcggc cgcaaaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
 1501 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
 1561 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
 1621 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
 1681 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
 1741 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
 1801 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
 1861 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
 1921 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
 1981 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
 2041 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
 2101 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
 2161 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
 2221 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
 2281 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
 2341 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
 2401 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
 2461 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgcat cgatggcccc
 2521 ccgatggtag tgtggggtct ccccatgcga gagtagggaa ctgccaggca tcaaataaaa
 2581 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
 2641 ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca acggcccgga
 2701 gggtggcggg caggacgccc gccataaact gccaggcatc aaattaagca gaaggccatc
 2761 ctgacggatg gcctttttgc gtggccagtg ccaagcttgc atgc
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