- ¥800 - 1200
- Ybscience
- 中国/美国
- YB-0137
- 2025年07月10日
企业认证
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
6个月
- 英文名：
pET-17b
- 库存：
大量
- 供应商：
钰博生物
- 规格：
1ug/5ug
pET17b载体基本信息
| 出品公司: | Ybscience |
|---|---|
| 别名: | pET17b, pet 17b |
| 质粒类型: | 大肠杆菌蛋白表达 |
| 表达水平: | 高 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 3306bp |
| 5' 测序引物: | T7 |
| 5' 测序引物序列: | 5'-TAATACGACTCACTATAGGG-3' |
| 载体标签: | N-T7 |
| 载体抗性: | Ampicillin |
| 备注: | Hosts: E.coli. Related vectors: pBR322. |
| 稳定性: | 瞬时表达 Transient |
| 组成型: | 组成型 Constitutive |
| 病毒/非病毒: | 非病毒 |
pET17b载体质粒图谱和多克隆位点信息
pET17b载体简介
The pET-17b vector (Cat. No. 69663-3) carries an N-terminal 11aa T7?Tag? sequence followed by a region of useful cloning sites. Included in the multiple cloning region are dual BstX I sites, which allow efficient cloning using an asymmetric linker. Unique sites (except for the two BstX I sites) are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circluar map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below.
pET17b载体序列
LOCUS pET-17b 3306 bp DNA circular SYN
DEFINITION pET-17b
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTOAUTHORAD1|Novagen Inc.|
FEATURES Location/Qualifiers
source 1..3306
/organism="pET-17b"
/mol_type="other DNA"
terminator complement(3..131)
/label="T7_terminator"
misc_feature 71..89
/label="T7_Terminal_primer"
misc_feature complement(237..269)
/label="T7_leader"
misc_feature complement(252..270)
/label="Xpress_fwd_primer"
misc_feature complement(278..294)
/label="T7_transl_en_RBS"
promoter complement(331..349)
/label="T7_promoter"
misc_feature 858..1049
/label="ROP"
misc_feature complement(1043..1065)
/label="pGEX_3_primer"
gene complement(2238..3098)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(2238..3098)
/label="ORF frame 2"
promoter complement(3140..3168)
/label="AmpR_promoter"
ORIGIN
1 ttatccggat atagttcctc ctttcagcaa aaaacccctc aagacccgtt tagaggcccc
61 aaggggttat gctagttatt gctcagcggt ggcagcagcc aactcagctt cctttcgggc
121 tttgttagca gccggatctg ctcgagcggc cgccagtgtg atggatatct gcagaattcc
181 agcacactgg cggccgttac tagtggatcc gagctcggta ccaagcttga atcccgaccc
241 atttgctgtc caccagtcat gctagccata tgtatatctc cttcttaaag ttaaacaaaa
301 ttatttctag agggaaaccg ttgtggtctc cctatagtga gtcgtattaa tttcgcggga
361 tcgagatctc gggcagcgtt gggtcctggc cacgggtgcg catgatcgtg ctcctgtcgt
421 tgaggacccg gctaggctgg cggggttgcc ttactggtta gcagaatgaa tcaccgatac
481 gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc gacctgagca acaacatgaa
541 tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg ccctgcacca
601 ttatgttccg gatctgcatc gcaggatgct gctggctacc ctgtggaaca cctacatctg
661 tattaacgaa gcgctggcat tgaccctgag tgatttttct ctggtcccgc cgcatccata
721 ccgccagttg tttaccctca caacgttcca gtaaccgggc atgttcatca tcagtaaccc
781 gtatcgtgag catcctctct cgtttcatcg gtatcattac ccccatgaac agaaatcccc
841 cttacacgga ggcatcagtg accaaacagg aaaaaaccgc ccttaacatg gcccgcttta
901 tcagaagcca gacattaacg cttctggaga aactcaacga gctggacgcg gatgaacagg
961 cagacatctg tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc tgcctcgcgc
1021 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
1081 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
1141 ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa
1201 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atatgcggtg tgaaataccg
1261 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac
1321 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
1381 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
1441 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
1501 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
1561 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
1621 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
1681 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
1741 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
1801 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
1861 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
1921 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
1981 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
2041 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
2101 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
2161 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
2221 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
2281 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
2341 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
2401 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
2461 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
2521 gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg
2581 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
2641 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
2701 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
2761 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
2821 atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc
2881 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
2941 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
3001 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
3061 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
3121 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
3181 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
3241 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt
3301 caagaa
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验, either alone or in combination with chemo- or radiotherapy at the more advanced stages. However, many patients present with metastatic disease at the time of diagnosis. Both computed tomography (CT) and positron emission tomography (PET) using fluorodeoxyglucose (FDG) play
什么是PET 树脂 ---PET是英文Polyethylene terephthalate的缩写，PET的中文意思是聚对苯二甲酸乙二醇酯。PET是以石油中的对苯二甲酸和乙二醇为原料，在高温高真空条件下进行化学反应而形成的树脂之一。PET瓶具有综合性能优良的特点，在饮料行业被广泛使用。 核医学 ---PET是英文Positron Emission Tomography的缩写，PET的中文意思是正电子发射计算机断层扫描。PET是核医学发展的一项新技术。 PET瓶片－－
PET and PET/CT in the Management of Thyroid Cancer
The introduction of PET(-CT) has brought about a major paradigm shift in the management of thyroid carcinoma, especially from the diagnostic standpoint. From the viewpoint of patient management, the areas where it has made significant impact
技术资料暂无技术资料 索取技术资料
