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Bioworld Technology,Inc.
1660 South Highway 100, Suite 500 St. Louis Park, MN 55416, USA
Tel: 612-326-3284
Fax:612-293-3841
Email: info@bioworlde.com
- 询价
- bioworld
- BSAP0060
- 2025年07月15日
- ,
- rabbit
- human
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 宿主：
rabbit
- 抗原：
兔
- 应用范围：
,
- 适应物种：
human
- 抗原来源：
rabbit
- 是否单克隆：
2
- 保存条件：
-20
- 规格：
25/50/100/200
β-Actin pAb
Product
1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.
Purification & Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Specificity
β -Actin antibody detects endogenous levels of β -Actin protein.
Applications (Recommended Dilutions)
WB: 1:3000~1:7000
Western Blot(WB)
Western blot (WB) analyzes of β-Actin pAb in extracts from Rat brain/Mouse raw cell/Hela cells.
Storage&Stability
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Background
All eukaryotic cells express actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of actin are present in mammalian tissues and fall into three classes. -actin expression is limited to various types of muscle, whereas - and -actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the actin cytoskeleton. Rho controls the assembly of ctin stress fibers and focal adhesion, Rac regulates ctin filament accumulation at the plasma membrane and Cdc42 stimulates formation of filopodia.
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文献和实验相关实验
mnidr 我想做拟南芥基因和烟草基因的NORTHERN，想选择β-actin作为内参，请问，在拟南芥和烟草中β-actin的序列是一样的吗？如果在更远的物种里呢，比如动物？？？？ freecell 脊椎动物中不同物种的β-actin基因氨基酸之间的序列同源性超过98%。其它种属不清楚，你可以在NCBI里面blast一下。 sherwing2009 可以在NCBI中，blast一下，相似
小鼠β-肌动蛋白(β-actin)酶联免疫分析（ELISA）
小鼠β- 肌动蛋白 ( β -actin) 酶联免疫 分析（ ELISA ） 试剂 盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定小鼠血清，血浆及相关液体样本中 β-肌动蛋白(β-actin) 含量。 实验原理 ： 本试剂盒应用双抗体夹心法测定 标本 中 小鼠β- 肌动蛋白 ( β -actin) 水平。用纯化的 小鼠β- 肌动蛋白 ( β -actin) 抗体包被微孔板，制成固相抗体，往包被单抗的微孔
Q1： 要做real-time pcr，而该种的β-actin未确定，我知道一般用持家基因作内参，但似乎其他基因各有缺点，有的作者用他们做内参时被SCI杂志要求用两个内参以证实，我想知道的是除了β-actin，哪个基因是SCI公认的内参基因(不用作两个内参了)? A1： 28S和18S rRNA 甘油醛-3-磷酸脱氢酶 还有α-tublin,ubiquitin,GAPDH,histone Q2： 做相对定量real
β-Actin
询价
