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PMA/Ionomycin mixture(250X)佛波酯

/离子霉素混合物
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  • ¥460 - 2060
  • MultiSciences已认证
  • 杭州
  • CS1001
  • 2026年04月09日
  • FCM(FACS)
  • NoData
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    • 详细信息
    • 用户评价
    • 文献和实验
    • 技术资料
    • 保存条件

      2 - 8℃避光,切勿冻存

    • 克隆性

      多克隆

    • 标记物

      NoData

    • 适应物种

      NoData

    • 供应商

      联科生物

    • 应用范围

      FCM(FACS)

    • 规格

      500ul/100ul

    规格:500ul产品价格:¥2060.0
    规格:100ul产品价格:¥460.0
    1.产品简介 
    PMA/Ionomycin Mixture(250×)是佛波酯(Phorbol 12-Myristate 13-Acetate, PMA)和离子霉素(ionomycin) 的混合物。PMA 和 ionomycin 处理足以诱导很多类型的细胞活化,分泌细胞因子。该混合物
    可用于刺激体外培养细胞产生细胞因子,随后用免疫学方法进行检测。还可以与蛋白转运抑制剂(BFA/Monensin Mixture (250×) (目录号 CS1002)联合使用,使诱导的细胞因子滞留在胞内。使用时,终
    浓度稀释至1×,即每250μl细胞培养基中加入1μl PMA/Ionomycin Mixture (250×)。
     
      
    2.产品特点 
    高品质原料:来自sigma; 
    使用方便,以浓缩液形式提供,无需繁琐计算,按比例加入即可; 
    均为液体,低温不会冻融,无需分装直接取用; 
    可以将刺激时间延长至18h,更容易检测,方便安排实验,可以过夜培养。 
      
    3.结果示例
    产品细节图片1
    本产品引用文献15篇,部分代表文献如下,全部文献引用请下载产品资料查看: 
      
    [1] Zhao J, Wang H, Dai C, et al. P2X7 blockade attenuates lupus nephritis by inhibiting NLRP3/ASC/caspase-1 activation[J]. Arthritis & Rheumatism, 2013 (IF=7.477) 
    [2] Liu Y, Chen Z, Xu K, et al. Next generation sequencing for miRNA profile of spleen CD4 +, T cells in the murine model of acute asthma [J]. Epigenomics, 2018 (IF=4.404) 
    [3] Xiaoxiang Li, Kui Xu, Wei Li, et al. Immunologic Response to Tumor Ablation with Irreversible Electroporation [J]. PLOS ONE, 2012, 7(11): e48749 (IF=4.092) 
    [4] Tong Bei, Dou Yannong, Wang Ting, et al. Norisoboldine ameliorates collagen-induced arthritis through regulating the balance between Th17 and regulatory T cells in gut-associated lymphoid tissues [J]. Toxicology and Applied Pharmacology, 2015, 282: 90-99 (IF=3.705) 
    [5] Shunlan N, Shanshan L, Naibin Y, et al. Deregulation of Regulatory T Cells in Acute-on-Chronic Liver Failure: A Rat Model [J]. Mediators of Inflammation, 2017, 2017: 1-10 (IF=3.418) 
    [6] Aijun J, Yueling W, Wenjin S, et al. MBD2 Regulates Th17 Cell Differentiation and Experimental Severe Asthma by Affecting IRF4 Expression[J]. Mediators of Inflammation, 2017, 2017: 1-10 (IF=3.232) 
    部分证书资料如下:







     

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        Phosphate Buffered Saline(PBS)(3)Wash Buffer:1×PBS,0.1%Tween-20(Santa cruz biotechnology,sc-29113)(4)Phorbol 12-myristate13-acetate(PMA)(Sigma 79346)(5)钙离子载体Ionomycin(SigmaI 9657) 二、设备 1. AID EliSpot Readerclassic(Autoimmun Diagnostika GmbH) 三

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      资料下载:

      CS1001引用文献.pdf 附 (下载 21 次)

      研选
      PMA/Ionomycin mixture(250X)佛波酯/离子霉素混合物
      ¥460 - 2060