Recombinant Human Carboxypepti

Recombinant Human Carboxypeptidase B2/CPB2  (CA32)

 

产品说明(Description)

Recombinant Human Carboxypeptidase B2 is produced by our Mammalian expression system and the target gene encoding Phe23-Val423 is expressed with a 6His tag at the C-terminus. 

Accession #: NP_001863.3

Known as: Carboxypeptidase B2; Carboxypeptidase U; CPU; Plasma Carboxypeptidase B; pCPB; Thrombin-Activable Fibrinolysis Inhibitor; TAFI; CPB2

 

制剂(Formulation)

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 1mM ZnCl₂, 10% Glycerol, pH 7.5.

 

质量控制(Quality Control) 

 

Purity:           Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin:    Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

 

 

运输(Shipping)

The product is shipped on dry ice/polar packs. 
Upon receipt, store it immediately at the temperature listed below.

 

保存(Storage)

Store at ≤-70°C, stable for 6 months after receipt.
Store at ≤-70°C, stable for 3 months under sterile conditions after opening. 
Please minimize freeze-thaw cycles.     

 

背景(Background)

Carboxypeptidase B2 (CPB2) is a secreted enzyme that belongs to the peptidase M14 family. CPB2 is synthesized by the liver and circulates in the plasma as a plasminogen-bound zymogen by the liver and circulates in the plasma as a plasminogen-bound zymogen. CPB2 cleaves C-terminal arginine or lysine residues from biologically active peptides, such as kinins or anaphylatoxins, in the circulation regulating their activities. CPB2 also down-regulates fibrinolysis by removing C-terminal lysine residues from fibrin that has already been partially degraded by plasmin. CPB2 exhibits carboxypeptidase activity when it is activated by proteolysis at residue Arg92 of the thrombin/thrombomodulin complex. Activated CPB2 reduces fibrinolysis by removing the fibrin C-terminal residues that are important for the binding and activation of plasminogen.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY