Recombinant Human B7-H4 (C-mFc

Recombinant Human B7-H4 (C-mFc) (CW23)

产品说明(Description)

Recombinant Human B7 Homolog 4 is produced by our Mammalian expression system and the target gene encoding Phe29-Ala258 is expressed with a mFc tag at the C-terminus. 

Accession #: Q7Z7D3

Known as: B7S1; B7x; Vtcn1; B7h.5; B7-H4; B7H4T-cell costimulatory molecule B7x; B7S1VCTN1; B7XPRO1291; FLJ22418; Immune costimulatory protein B7-H4; Protein B7S1; T cell costimulatory molecule B7x; V-set domain containing T cell activation inhibitor 1; V-set domain-containing T-cell activation inhibitor 1
 

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
 

质量控制(Quality Control) 

 

Purity:        Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin: Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test.

Bioactivity-Elisa:

 

Immobilized Human B7-H4-mFc(Cat#CW23) at 2μg/ml (100 μl/well) can bind Anti-Human B7-H4 mAb(Cat#NC054). The ED50 of Anti-Human B7-H4 mAb(Cat#NC054) is 6.24 ng/ml.	------	Immobilized Human B7-H4-mFc(Cat#CW23) at 2μg/ml (100 μl/well) can bind Anti-Human B7-H4 mAb(Cat#NC054). The ED50 of Anti-Human B7-H4 mAb(Cat#NC054) is 6.24 ng/ml.

 

复溶(Reconstitution)

Dissolve the lyophilized protein in distilled water.    

 

保存(Storage)

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.

Reconstituted protein solution can be stored at 4-7°C for 2-7 days.

Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景(Background)

B7 Homolog 4 (B7-H4) is glycosylated member of the B7 family of immune costimulatory proteins.  Mature human B7-H4 consists of a 235 amino acid (aa) extracellular domain (ECD) with two Ig-like V-type domains, a 21 aa transmembrane segment, and a 2 aa cytoplasmic tail. It is widely expressed, including in kidney, liver, lung, pancreas, placenta, prostate, spleen, testis and thymus. B7-H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. It also involved in promoting epithelial cell transformation.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY