Recombinant Human IFN-gamma/IF

Recombinant Human IFN-gamma/IFNγ  (CI57)

 

产品说明(Description)

Recombinant Human Interferon gamma is produced by our Mammalian expression system and the target gene encoding Gln24-Gln166 is expressed. 

Accession #: P01579

Known as: Interferon Gamma; IFN-Gamma; Immune Interferon; IFNG

 

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of 4mM HCl.

 

质量控制(Quality Control)

Purity:           Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin:    Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

 

复溶(Reconstitution)

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存(Storage)

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.

Reconstituted protein solution can be stored at 4-7°C for 2-7 days.

Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景(Background)

IFNγ is the major interferon produced by mitogenically or antigenically stimulated lymphocytes. It is structurally different from type I interferon and its major activity is immunoregulation. It has been implicated in the expression of class II histocompatibility antigens in cells that do not normally produce them, leading to autoimmune disease. Interferon gamma is produced mainly byT-cells and natural killer cells activated by antigens, mitogens, or alloantigens. It is produced by lymphocytes expressing the surface antigens CD4 and CD8. IFNγ synthesis is induced by IL-2, FGF-basic, and EGF.

 

电泳(SDS-PAGE)

 

参考文献(Reference)

Selection of a Novel DNA Aptamer for Assay of Intracellular Interferon-Gamma. Cao B. et al. PloS one. 2014.

 

 

FOR RESEARCH USE ONLY