Recombinant Human IFN-gamma

Recombinant Human IFN-gamma (C014)

产品说明(Description)

Recombinant Human Interferon gamma is produced by our E.coli expression system and the target gene encoding Gln24-Gln166 is expressed.

Accession #: P01579

Known as: Interferon Gamma; IFN-Gamma; Immune Interferon; IFNG

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 250mM NaCl, 5% Trehalose, pH 8.5.

质量控制(Quality Control)

Purity: Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin: Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test.

Bioactivity: Measured by a cytotoxicity assay using HT-29 cells. The ED50 for this effect is 17 pg/mL.

复溶(Reconstitution)

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

保存(Storage)

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

背景(Background)

IFNγ is the major interferon produced by mitogenically or antigenically stimulated lymphocytes. It is structurally different from type I interferon and its major activity is immunoregulation. It has been implicated in the expression of class II histocompatibility antigens in cells that do not normally produce them, leading to autoimmune disease. Interferon gamma is produced mainly byT-cells and natural killer cells activated by antigens, mitogens, or alloantigens. It is produced by lymphocytes expressing the surface antigens CD4 and CD8. IFNγ synthesis is induced by IL-2, FGF-basic, and EGF.

电泳(SDS-PAGE)

参考文献(References)

Target-triggered programming of cascaded catalytic hairpin assemblies for enzyme-free and highly sensitive sensing of cytokines[J]. Sujing Wang. et al. Sensors and Actuators B-Chemical. 2021.

Ultrasensitive aptamer fluorometric detection of IFN-γ by dual atom transfer radical polymerization amplification[J]. DongxiaoWen. et al. Sensors and Actuators B: Chemical. 2019.

Discovery of hPRDX5‑based peptide inhibitors blocking PD‑1/PD‑L1 interaction through in silico proteolysis and rational design[J]. Sen Zou. et al. Cancer Chemotherapy and Pharmacology. 2019.

Phenotype and biological characteristics of endometrial mesenchymal stem / stromal cells：A comparison between intrauterine adhesion patients and healthy women[J]. Jiali Min. et al. American Journal of Reproductive Immunology. 2020.

Original Article Efficacy analysis of triple-negative breast cancer patients treated with dendritic cell-cytokine-induced killer cell immunotherapy[J]. Yunqing Xie. et al. International Journal of Clinical and Experimental Medicine. 2019.

Influence of the number and interval of treatment cycles on cytokineinduced killer cells and their adjuvant therapeutic effects in advanced nonsmall-cell lung cancer (NSCLC)[J]. Yuanlong Gu. et al. International Immunopharmacology. 2017.

FOR RESEARCH USE ONLY