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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
美国
- 亚型:
100µl
- 形态:
W IP F
- 保存条件:
-20C
- 克隆性:
多克隆
- 浓度:
DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody)
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文献和实验Studies of the Ubiquitin Proteasome System
from other investigators) Transfection efficiency control plasmid, e.g., GFP, RFP, β‐galactosidase 2× HEPES‐buffered saline (HeBS; see recipe ) Anti– tag epitope antibody or anti‐substrate
Quantitative immunoprecipitation of GFP-fusion proteins using the GFP-Trap
with a small protein tag (e.g. Histidine-tag, c-Myc, FLAG or hemaglutinin). GFP, the most widely used labelling tag in cell biology is rarely used for biochemical analyses although various mono- and polyclonal antibodies are available (Cristea et al., 2005
40 concentrations (see reciperecipes ) Protein G–agarose (Roche, cat. no. 11‐243‐233001) FLAG M2‐agarose beads (Sigma, cat
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