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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
常温运输,室温密封保存
- 保质期:
一年
- 英文名:
Methanol
- 库存:
大量
- 供应商:
上海钰博生物科技有限公司
- 规格:
500 mL/盒
别名:木醇;木精。分子式:CH3OH,分子量:32.04。应用:有机合成的甲基化试剂;防冻剂;萃取剂;脱水剂;浸出剂。
外观:无色澄清液体沸点:64.7℃熔点:-98℃密度:0.791g/mL at 25℃
常温运输,室温密封保存,有效期一年。BCA快速蛋白定量试剂盒 500T
Bradford快速蛋白定量试剂盒 500T
蛋白质银染试剂盒 100ml
SDS-page胶制备试剂盒 50T
SDS-page胶制备试剂盒 100T
快速考马斯亮蓝染色液(G-250型) 100ml
甲醇-Methanol快速考马斯亮蓝染色液(R-250型) 100ml
考马斯亮蓝染色液(常规法) 250ml
考马斯亮蓝染色脱色液(常规法) 500ml
丽春红染色液 120ml
ECL底物发光试剂盒(试剂A:25ml,试剂B:25ml) 50ml
ECL底物发光试剂盒(试剂A:50ml,试剂B:50ml) 100ml
可溶型TMB显色底物试剂 100ml
可溶型TMB显色底物试剂 500ml
沉淀型TMB显色底物试剂 100ml
沉淀型TMB显色底物试剂 500ml
HRP-DAB底物显色试剂盒 60ml
超敏可逆蛋白显色试剂 100ml
碱性磷酸酶底物显色试剂盒(BCIP/NBT) 100 ml
胞浆总蛋白提取试剂盒 50T
植物细胞质膜提取试剂盒 50T
植物蛋白提取试剂盒 50T
细菌总蛋白提取试剂盒 50T
酵母总蛋白提取试剂盒 50T
胞浆和核蛋白分步提取试剂盒 50T
膜蛋白提取试剂盒 50T
动物组织蛋白裂解液 50ml
真核细胞蛋白裂解液 50ml
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文献和实验Methanol Fixation for Immunofluorescence
Methanol fixation works by precipitating proteins, and as such it is a quick method (2-5)minutes is enough time for most antibodies/proteins). Diffuse proteins may be lost,however, in which case PFA fixation may be a better choice
Yeast IF with Methanol/Acetone Dehydration
into another coplin jar that contains acetone and is also surrounded by dry ice for 30sec. It is important that the jars and contents are down to temperature so place them on the dry ice an hour or two before this step. Change your methanol and acetone frequently
Yeast IF with Methanol/Acetone Dehydration
Yeast IF with Methanol/Acetone Dehydration David Amberg Note this protocol is a modified version of the protocol from Mark Rose in the CSH Yeast Genetics Course Manual. This is the protocol to use with the Botstein anti-actin
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