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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ 至 -80℃
- 保质期:
12个月
- 英文名:
Influenza A H3N2 (A/Wyoming/03/2003) Hemagglutinin / HA Protein (His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
100 µg
蛋白名称:Hemagglutinin/HA蛋白, Hemagglutinin/HA protein
蛋白构建:A DNA sequence encoding the extracellular domain (Met 1-Trp 530) of the influenza A H3N2 hemagglutinin (A/Wyoming/03/2003 (H3N2)) (ABX10525.1) (HA1+HA2, uncleaved) was expressed, fused with a C-terminal polyhistidine tag.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE.
蛋白活性:Measured by its ability to agglutinate guinea pig red blood cells. HA titer is 4-20 μg/mL for 1% GRBC.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Gln 17
蛋白分子量:The secreted recombinant influenza A H3N2 HA (A/Wyoming/03/2003 (H3N2)) comprises 525 amino acids and has a predicted molecular mass of 59 kDa. As a result of glycosylation, it migrates as an approximately 80-90 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号:ABX10525.1
蛋白氨基酸序列:Met1-Trp530
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验PCR for the Detection of Influenza Viruses in Clinical Material
). Influenza A viruses are further divided into subtypes H1N1, H2N2 and H3N2, based on differences in the hemagglutinin (HA) and neuraminidase (NA) genes. The ability of influenza viruses to undergo antigenic change in HA and NA enables the virus to evade
Genetic Engineering of Live Attenuated Influenza Viruses
The first live attenuated influenza vaccine (LAIV) was licensed in the USA in 2003; it is a trivalent vaccine composed of two type A (H1N1 and H3N2) and one type B influenza virus each at 107 fluorescent focus units (FFU). Each influenza
酶解再向β折叠链的构象转变成无活力的裂解型(cleaved-form, C型)[11]. L型抑制剂可以通过变性和复性方法转变成A型, 然后又慢慢变成L型. 流感病毒血细胞凝集素(influenza hemagglutinin, HA)在pH诱导下的结构变化与细胞膜融合功能紧密相关的. HA在pH 7.0时, 其空间结构是由helix-loop-helix的头状单体结构域组成的三聚体蛋白(HA-N); 可在低pH(pH<5)条件下, 中间Loop区域转化为螺旋结构并与另两段螺旋连接共同形成一股长螺旋
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