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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
在-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Human Carbonic Anhydrase III / CA3 Protein (His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
100 µg/1 mg
| 规格: | 100 µg | 产品价格: | ¥3220.0 |
|---|---|---|---|
| 规格: | 1 mg | 产品价格: | ¥25190.0 |
蛋白名称:Human Carbonic Anhydrase III / CA3 Protein (His Tag)
蛋白构建:A DNA sequence encoding the human CA3 (NP_005172.1) (Met 1-Lys 260) was expressed, with a polyhistide tag at the C-terminus.
表达宿主:E. coli
蛋白纯度:> 95 % as determined by SDS-PAGE
蛋白活性:Measured by its esterase activity. The specific activity is >5 pmoles/min/μg.
蛋白内毒素:Please contact us for more information.
预测N端:Met 1
蛋白分子量:The recombinant human CA3 consisting of 266 amino acids and has a calculated molecular mass of 30.4 kDa as estimated in SDS-PAGE under reducing conditions.
蛋白NP号:NP_005172.1
蛋白氨基酸序列:Met1-Lys260
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Strategies to Optimize Protein Expression in E. coli
Peng, L., Xu, Z.N., Fang, X.M., Wang, F., Yang, S., and Cen, P.L. 2004. Preferential codons enhancing the expression level of human beta‐defensin‐2 in recombinant Escherichia coli. Protein Pept. Lett. 11:339
carbonic anhydrase B, J. Biol. Chem. 267 (1992) 13327-13334. 51. X.D. Geng, X.Q. Chang, High performance hydrophobic interaction chromatography as a tool for protein refolding, J. Chromatogr. A 599 (1992) 185-194. 52. J.D. Carlson, M.L. Yarmush
components, and other miscellaneous glycoproteins. In the kidney, several GPI-anchored proteins have been identified, including uromodulin (Tamm-Horsfall glycoprotein), carbonic anhydrase type IV, alkaline phosphatase, Thy-1, BP-3, aminopeptidase P
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