Recombinant Human Carbonic Anhydrase II / CA2 Protein (His Tag) | 重组人 Carbonic Anhydrase II / CA2 蛋白 (His标签)
- ￥2570 - 16740
- Sino Biological
- 北京
- 10478-H08E
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
在-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human Carbonic Anhydrase II / CA2 Protein (His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
100 µg/1 mg
| 规格： | 100 µg | 产品价格： | ￥2570.0 |
|---|---|---|---|
| 规格： | 1 mg | 产品价格： | ￥16740.0 |
蛋白名称：Human Carbonic Anhydrase II / CA2 Protein (His Tag)
蛋白构建：A DNA sequence encoding the human CA2 (NP_000058.1) (Met 1-Lys 260) was expressed, with a polyhistide tag at the C-terminus.
表达宿主：E. coli
蛋白纯度：> 96 % as determined by SDS-PAGE
蛋白活性：Measured by its esterase activity. The specific activity is >150 pmoles/min/μg .
蛋白内毒素：Please contact us for more information.
预测N端：Met 1
蛋白分子量：The recombinant human CA2 consisting of 266 amino acids and has a calculated molecular mass of 30 kDa as estimated in SDS-PAGE under reducing conditions.
蛋白NP号：NP_000058.1
蛋白氨基酸序列：Met1-Lys260
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, Zhang, ZP; et al. Synthesis, Molecular Docking Analysis, and Carbonic Anhydrase Inhibitory Evaluations of Benzenesulfonamide Derivatives Containing Thiazolidinone. Molecules, Pubmed: 31262068
3, Zhang, J; et al. 5-[2-(N-(substituted phenyl) acetamide)] amino-1, 3, 4-thiadiazole -2-sulfonamides as selective carbonic anhydrase II inhibitor with neuroprotective effects. ChemMedChem, Pubmed: 32141184
4, Bartoschik, TA. Development and validation of new approaches for quantitative intermolecular interaction analysis by MicroScale Thermophoresis under near-native experimental. Thesis, Pubmed: 31214999
Purification of Human Multiprotein Complexes using OneSTrEP Technology (PROT41)
®II (SAWRHPQFGG) and its “double” sister, the One-STrEP-tag (tandem arrangement of Strep -tag®II, here called OneStrep), are reasonably small protein tags that usually do not influence protein folding and function. However, always check
Introduction of human 14-3-3 proteins
of tyrosine and tryptophan hydroxylases and for inhibition of Ca2+/phospholipid-dependent protein kinase C.Northern blot analysis demonstrated widespread expression of the 14-3-3 protein eta chain in cultured cell lines derived from various human tumors
Strategies to Optimize Protein Expression in E. coli
Peng, L., Xu, Z.N., Fang, X.M., Wang, F., Yang, S., and Cen, P.L. 2004. Preferential codons enhancing the expression level of human beta‐defensin‐2 in recombinant Escherichia coli. Protein Pept. Lett. 11:339
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