- ￥2980 - 6880
- Sino Biological
- 北京
- 10389-H27H-B
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human CD16a/FCGR3A Protein(176 Phe,His&AVI Tag),Biotinylated
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
20.00 µg/50.00 µg/100.00 µg
| 规格： | 20.00 µg | 产品价格： | ￥2980.0 |
|---|---|---|---|
| 规格： | 50.00 µg | 产品价格： | ￥4520.0 |
| 规格： | 100.00 µg | 产品价格： | ￥6880.0 |
人 CD16a蛋白|CD16a protein|CD16a(Human, C-AVI & His)（产品说明）
蛋白名称：CD16a蛋白, CD16a protein
蛋白构建：A DNA sequence encoding the extracellular domain (Met 1-Gln 208) of human CD16a (P08637-1) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主：HEK293 Cells
蛋白纯度：> 90 % as determined by SDS-PAGE
蛋白活性：1. Measured by its ability to bind recombinant human IgG1 in a functional ELISA. 2. Measured by its binding ability in a functional ELISA. Immobilized Human IgG1 at 10 μg/ml (100 μl/well) can bind Human CD16a(Cat:10389-H27H-B). The EC50 of Human CD16a(Cat:10389-H27H-B) is 2.4 - 5.7 μg/ml. 3. Using the Octet RED System, the affinity constant (Kd) of CD16a Protein, Human, Recombinant (176 Phe, His & AVI Tag), Biotinylated (Cat. 10389-H27H-B) bound to humira was 0.5 μM.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Gly 17
蛋白分子量：The secreted recombinant human CD16a consists of 223 amino acids and predicts a molecular mass of 25.1 kDa. By SDS-PAGE under reducing conditions, the apparent molecular mass of the protein is approximately 48 kDa due to glycosylation.
蛋白NP号：P08637-1
蛋白氨基酸序列：Met1-Gln208
蛋白标签：C-AVI & His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
蛋白名称：CD16a蛋白, CD16a protein
蛋白构建：A DNA sequence encoding the extracellular domain (Met 1-Gln 208) of human CD16a (P08637-1) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主：HEK293 Cells
蛋白纯度：> 90 % as determined by SDS-PAGE
蛋白活性：1. Measured by its ability to bind recombinant human IgG1 in a functional ELISA. 2. Measured by its binding ability in a functional ELISA. Immobilized Human IgG1 at 10 μg/ml (100 μl/well) can bind Human CD16a(Cat:10389-H27H-B). The EC50 of Human CD16a(Cat:10389-H27H-B) is 2.4 - 5.7 μg/ml. 3. Using the Octet RED System, the affinity constant (Kd) of CD16a Protein, Human, Recombinant (176 Phe, His & AVI Tag), Biotinylated (Cat. 10389-H27H-B) bound to humira was 0.5 μM.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Gly 17
蛋白分子量：The secreted recombinant human CD16a consists of 223 amino acids and predicts a molecular mass of 25.1 kDa. By SDS-PAGE under reducing conditions, the apparent molecular mass of the protein is approximately 48 kDa due to glycosylation.
蛋白NP号：P08637-1
蛋白氨基酸序列：Met1-Gln208
蛋白标签：C-AVI & His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验该产品被引用文献
1, Bruggeman CW, et al. Enhanced Effector Functions Due to Antibody Defucosylation Depend on the Effector Cell Fcγ Receptor Profile.Journal of immunology (Baltimore, Md. : 1950), PubMed ID: 28566370
2, Temming AR, et al. Cross-reactivity of mouse IgG subclasses to human Fc gamma receptors: Antibody deglycosylation only eliminates IgG2b binding.Molecular immunology, PubMed ID: 32947169
2, Temming AR, et al. Cross-reactivity of mouse IgG subclasses to human Fc gamma receptors: Antibody deglycosylation only eliminates IgG2b binding.Molecular immunology, PubMed ID: 32947169
相关实验
连接就需要应用较强的增强组织通透性的试剂。增强组织通透性常用的方法如应用稀释的酸洗涤、去垢剂（detergent）或称清洗剂Triton X-100、酒精或某些消化酶如胃蛋白酶、胰蛋白酶、胶原蛋白酶和淀粉酶（diastase）等。这种广泛的去蛋白作用无疑可增强组织的通透性和核酸探针的穿透性，提高杂交信号，但同时也会减低RNA的保存最和影响组织结构的形态，因此，在用量及孵育时间上应慎为掌握。 蛋白酶K（Proteinase K）的消化作用在ISHH中是应用于蛋白消化的关键步骤，其浓度及孵育
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文献支持
Sino Biological CD16a/FCGR3A重组蛋白 His&AVI标签 生物素化
￥2980 - 6880
