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- 规格:
100 µg
蛋白名称:Cystatin S蛋白, Cystatin S protein
蛋白构建:A DNA sequence encoding the human CST4 (NP_001890.1) (Met 1-Ala 141) was fused with a polyhistidine tag at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 98 % as determined by SDS-PAGE
蛋白活性:0
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Ser 21
蛋白分子量:The secreted recombinant human CST4 consists of 132 amino acids and migrates as an approximately 16 kDa band in SDS-PAGE under reducing conditions as predicted.
蛋白NP号:NP_001890.1
蛋白氨基酸序列:Met1-Ala141
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Analysis of the Protein S Gene in Protein S Deficiency
Protein S (PS) is a 71-kDa vitamin K-dependent glycoprotein first identified in human plasma by DiScipio and colleagues in 1977 (1 ), a year after the discovery of the anticoagulant protein C (PC) (2 ,3 ). A few years later, Walker
Protein S (PS) is a vitamin K-dependent plasma glycoprotein. Around 60–70% of PS in plasma is noncovalently bound to C4-binding protein (C4BP). Free PS functions as a cofactor that enhances the activity of activated protein C (APC
Phosphoamino acid analysis:Mark Kamps's method
Recipes for the buffers are at the end of this protocol. 1.Label your protein with 32Pi.Then subject the protein to SDS polyacrylamide gel electrophoresis and transfer your gel-fractionated protein to Immobilon-P. Neither nitrocellulose nor nylon
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