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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
低温
- 英文名:
SDS (SODIUM DODECYL SULFATE), 10% SOLUTION
- 库存:
库存现货
- 供应商:
联硕
- CAS号:
477-73-6
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文献和实验完整蛋白质从 SDS-PAGE 胶中的电洗脱及其 MALDI-TOF MS 分析
analysis by mass spectrometry: principles, problems, pitfalls and recent developments. Phytochemistry65, 1449-1485. 3. Cohen, S. L. and Chait, B. T. (1997) M a s s spectrometry of whole proteins eluted from sodium dodecyl sulfate-polyacrylamide gel
transfers. For gels to be stained, stain for 20 min with Coomassie blue solution (see below), then destain in strong destain (50% methanol, 10% acetic acid) 20 min and leave in 10% acetic acid.Reagents for SDS PAGEStock solutions:A. Acrylamide=30 g
if gels of different size are used Fix the gel in 50% methanol and 10% acetic acid for 2 h with one change of the fixing solution after 1 h (gel may be left in the second change fixing solution overnight) 30 min in 5% methanol and 7% acetic
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