货号：VWRVE719 AMRESCO SDS 10%

Silver Staining SDS PAGE Gels

+ 30 g of NaOH 14.8 M Ammonium Hydroxide 38% Formaldehyde Ultra-pure water, use this for all steps and reagents 50% Aqueous Glutaraldehyde (optional) Glass tray or Novex Stain Ease Gel tray. If using glass, make sure

Tricine-SDS-PAGE

.   Cathode buffer (0.1 M Tris, 0.1 M tricine, 0.1% SDS, pH 8.25) Dissolve 6.055 g Tris base and 8.96 g tricine in a total volume of 500 ml dH2O. Filter the solution through a 0.45 µm filter, add 0.5 g electrophoresis-grade SDS

SDS-page minigel

can be made by dissolving 15.1 g of Tris base and 94 g of glycine in 900 ml of deionized H2 O. Then, 50ml of a 10% (w/v) stock solution of electrophoresis-grade SDS is added, and the volume is adjusted to 1000 ml withH2 O 3. Resolving gel 4X buffer 1.4M Tris