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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件:
干冰运输,-80℃保存
- 保质期:
6个月
- 英文名:
DH5α Competent Cell
- 库存:
大量
- 供应商:
上海钰博生物科技有限公司
- 规格:
0.1 mL×10/盒
种,具有下列特点:
DH5α感受态细胞-DH5α Competent Cell
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文献和实验CCMB buffer to make chemical competent cell
TOP10 chemically competent cells ( modified from http://openwetware.org/wiki/TOP10_chemically_competent_cells#CCMB80_buffer) I also use this protocol to make Ecoli compent cell. It is pretty good. You can start from the fresh clone on plate
实验概要 Competent cells are those that possess more easily altered cell walls that DNA can be passed through easily. These cells readily incorporate foreign DNA. On example of a competent cell is E. coli.
TOP10 chemically competent cells
Detergent is a major inhibitor of competent cell growth and transformation. Glass and plastic must be detergent free for these protocols. The easiest way to do this is to avoid washing glassware, and simply rinse it out. Autoclaving glassware filled
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