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低温
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大量
- 供应商:
上海研谨生物科技有限公司
- 规格:
B4252-100MG
产品名称【X-GAL 】
上海研谨生物公司是专业从事生化试剂、分子生物学试剂、实验耗材及实验仪器研发、销售、服务为一体的专业性生物科技公司目前公司已销售、代理:Amersham、sigma、Aldrich、Amresco 、YOYOBIO、BD(Difco)、Fluka、Merck、Omega、PALL、Millipore、Peprotech、Pierce、Roche、Eppendorf、 BioHiT、Whatman、Axygen、corning、Pharmaia、等诸多品牌产品并建立紧密的合作伙伴关系,代理其领先世界的尖端产品。
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文献和实验, transfect the DNA into mammalian cells, and then fix and stain a tissue with X-gal. The cells that turn blue must have the beta galactosidase enzyme, the product of the lac Z gene, which could only have been expressed from the donated promoter. Ergo
Materials Fixative 2% Paraformaldehyde (in PBS) Staining solution 5 mM potassium ferricyanide, 5 mM potassium ferrocyanide, 2 mM MgCI, 0.01% sodium deoxycholate 0.02% Nonidet P-40 (NP-40) in PBS. X-gal Stock 40 mg
Detection of -Galactosidase Activity: X-gal Staining
X-gal staining is a rapid and convenient histochemical technique used to detect reporter gene expression. A prerequisite is the creation or acquisition of transgenic reporter mouse lines, in which the bacterial LacZ gene has been knocked
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