- ¥800 - 1380
- HZbscience
- 中国/美国/德国
- HZ0291
- 2025年07月15日
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- 详细信息
- 技术资料
- 保存条件：
-20℃
- 保质期：
半年
- 英文名：
pFastBac1
- 库存：
200
- 供应商：
沪震生物
- 规格：
5μg
pFastBac1
产品信息
| 产品货号 | 产品名称 | 产品规格 | 优惠价 |
|---|---|---|---|
| HZ0291 | pFastBac1 | 20μl |
¥请询价 |
使用说明
沪震质粒平台的各批次质粒菌株发货前均经过严格的多重验证，如存在质量问题，请在收到产品的三个月内通知我司。收到质粒后请短暂离心，取2μl转化至对应感受态中，挑取单克隆重新提取质粒后使用。
基本信息
| 复制子: | pUC ori，F1 ori |
|---|---|
| 终止子: | SV40 poly(A) signal |
| 质粒分类: | 广宿主系列，昆虫细胞载体 |
| 质粒大小: | 4776bp |
| 原核抗性: | Amp |
| 筛选标记: | Gen |
| 克隆菌株: | DH5α |
| 培养条件: | 37℃，有氧 LB |
| 表达宿主: | 昆虫细胞 |
| 诱导方式: | 无须诱导，瞬时表达 |
| 5'测序引物: | pFastbac-F: TATTCCGGATTATTCATACC |
| 3'测序引物: | pFastBac-R: ACAAATGTGGTATGGCTGA |
质粒简介
pFastBac1载体是非融合性蛋白表达载体，在载体内部不带有其他融合性的蛋白标签。为了保证目的基因的正确表达，插入片段必须包含ATG起始密码子，保证蛋白起始翻译。插入片段最好包含终止密码子，保证蛋白质不会翻译出多余的氨基酸。在pFastBacI载体多克隆位点中，对于所有的三种读码框分布，都包含有终止密码子。
The Bac-to-Bac® Vector Kit contains a pFastBac™ 1 vector, as well an expression control vector, intended for use as part of the Bac-to-Bac® Baculovirus Expression System , which enables the efficent production of recombinant baculovirus for expression testing in insect cells. The Bac-to-Bac® System relies on generation of recombinant baculovirus by site-specific transposition in E. colirather than homologous recombination in insect cells (Figure 1). This system features:
Time-saving expression bacmid. With Bac-to-Bac®, the expression cassette of the pFastBac™ vector recombines with the parent bacmid in DH10Bac™ E. coli Competent Cells (not included with this vector kit) to form an expression bacmid. The bacmid is then transfected into insect cells for production of recombinant baculovirus particles.
Easy colony screening. The parent bacmid in DH10Bac™ E. colicontains a segment of the lacZα gene. The lacZα gene is disrupted upon transposition of the expression cassette into the bacmid allowing for blue/white selection of recombinants. This makes identification of recombinant colonies easy.
The Bac-to-Bac® Vector Kit contains a pFastBac™ 1 vector, as well an expression control vector, intended for use as part of the Bac-to-Bac® Baculovirus Expression System , which enables the efficent production of recombinant baculovirus for expression testing in insect cells. The Bac-to-Bac® System relies on generation of recombinant baculovirus by site-specific transposition in E. colirather than homologous recombination in insect cells (Figure 1). This system features:
Time-saving expression bacmid. With Bac-to-Bac®, the expression cassette of the pFastBac™ vector recombines with the parent bacmid in DH10Bac™ E. coli Competent Cells (not included with this vector kit) to form an expression bacmid. The bacmid is then transfected into insect cells for production of recombinant baculovirus particles.
Easy colony screening. The parent bacmid in DH10Bac™ E. colicontains a segment of the lacZα gene. The lacZα gene is disrupted upon transposition of the expression cassette into the bacmid allowing for blue/white selection of recombinants. This makes identification of recombinant colonies easy.
质粒图谱
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载体pFastBac1
¥800 - 1380
