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25T
产品编号:TBDCS025153
产品名称:DNA Damage Histone H2A.X Dual Detection Kit
产品规格:25T
产品价格:¥询价元
组蛋白H2A.X DNA损伤双向检测试剂盒
Histone H2A.X DNA Damage Dual Detection kit includes two directly conjugated antibodies, a phospho-specific Anti-phospho-Histone H2A.X (Ser139)-PerCP and an Anti-Histone H2A.X-FITC conjugated antibody to measure total levels of Histone H2A.X. This two color kit is designed to detect the extent of Histone H2A.X pathway activation by measuring H2A.X phosphorylation relative to the total H2A.X expression in any given cell population. By doing such, the levels of both the total and phosphorylated protein can be measured simultaneously in the same cell, resulting in a normalized and accurate measurement of H2A.X activation after stimulation. Moreover, simultaneous measurement of both total and phospho-Histone H2A.X confirms target specificity of the phosphorylation event. Together, a total and phospho antibody duo performed in multiplex provides an enhanced and more reliable detection of the phospho: total ratio within a mixed 2 cell population. Using this antibody pair provides a sensitive and valuable tool to study the factors that induce DNA damage and/or affect DNA repair, and allow one to explore the linkage between DNA damage, cell cycle checkpoints, and initiation of apoptosis.
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文献和实验配对间的氢键改变,可使腺嘌呤能配上胞嘧啶、胸腺嘧啶能配上鸟嘌呤等,如果这些配对发生在DNA复制时,就会造成子代DNA序列与亲代DNA不同的错误性损伤,如图16-18所示。 图16-18 腺嘌呤的稀有互变异体与胞嘧啶(a),或胸腺嘧啶的稀有互变异构体与鸟嘌呤� (b)的氢链形成导致下一世代中G-C配对取代A-T配对 b.碱基的脱氨基作用 碱基的环外氨基有时会自发脱落,从而胞嘧啶会变成尿嘧啶、腺嘌呤会变成次黄嘌呤(H)、鸟嘌呤会变成黄嘌呤(X)等,遇到复制时,U与A配对
网络 第三节 DNA的损伤与修复 DNA存储着生物体赖以生存和繁衍的遗传信息,因此维护DNA分子的完整性对细胞至关重要。外界环境和生物体内部的因素都经常会导致DNA分子的损伤或改变,而且与RNA及蛋白质可以在胞内大量合成不同,一般在一个原核细胞中只有一份DNA,在真核二倍体细胞中相同的DNA也只有一对,如果DNA的损伤或遗传
佚名 DNA存储着生物体赖以生存和繁衍的遗传信息,因此维护DNA分子的完整性对细胞至关重要。外界环境和生物体内部的因素都经常会导致DNA分子的损伤或改变,而且与RNA及蛋白质可以在胞内大量合成不同,一般在一个原核细胞中只有一份DNA,在真核二倍体细胞中相同的DNA也只有一对,如果DNA的损伤或遗传信息的改变不能更正,对体细胞就可能影响其功能或生存,对生
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