We have developed a multi-parameter flow cytometry assay for identification of mouse regulatory B cells from mouse splenocytes.TBD Mouse Breg Identification Kit includes three directly conjugated antibodies: Anti-Mouse CD5-APC, Anti-Mouse CD19-FITC, and Anti-Mouse CD1d-PE, along with optimized assay buffers and a blocking antibody to provide researchers the ability to phenotypically distinguish cell types.