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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量供应
- 供应商:
上海研谨生物
- 规格:
200ml/瓶
产品编号:TBD0002HY
产品名称:水饱和酚/200ml
产品规格:200ml/瓶
产品价格:¥120.00元
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文献和实验Alternate Sporulation Method for BY4743
10% Potassium Acetate (20g/200ml) 20 mls 0.005% Zinc Acetate 0.5% Zinc acetate (1g/200ml) 2 mls 1 x ura supplement 100 x ura
How to Make Simple Solutions and Dilutions
= 200 ul = 0.2 ml C2 = the new concentration = 25 mg/ ml By algebraic rearrangement: V1 = (V2 x C2) / C1 V1 = (0.2 ml x 25 mg/ml) / 100 mg/ml and after cancelling the units, V1 = 0.05 ml, or 50 ul
at 30 °C (shaking or not shaking) add 85 µl DMSO heat shock at 42 °C for 7 min spin, resuspend cells in 1 ml 1 x TE spin, resuspend cells in 0.5 ml 1 x TE plate 100 - 200 µl / plate and incubate at 30 °C
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