- ¥800 - 1380
- HZbscience
- 中国/美国/德国
- HZ0157
- 2025年07月11日
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- 详细信息
- 技术资料
- 保存条件：
-20℃
- 保质期：
半年
- 英文名：
pCDNA3.1(+)
- 库存：
200
- 供应商：
沪震生物
- 规格：
5ug
pCDNA3.1(+)
产品信息
| 产品货号 | 产品名称 | 产品规格 | 优惠价 |
|---|---|---|---|
| HZ0157 | pCDNA3.1(+) | 5μg |
¥请询价 |
使用说明
沪震质粒平台的各批次质粒菌株发货前均经过严格的多重验证，如存在质量问题，请在收到产品的三个月内通知我司。收到质粒后请短暂离心，取2μl转化至对应感受态中，挑取单克隆重新提取质粒后使用。
基本信息
| 启动子: | CMV promoter |
|---|---|
| 复制子: | SV40 ori，pUC ori，f1 ori |
| 终止子: | BGH poly(A) signal |
| 质粒分类: | 哺乳系列质粒；哺乳表达质粒；pCDNA系列质粒 |
| 质粒大小: | 5428bp |
| 原核抗性: | 氨苄青霉素Amp（100μg/ml） |
| 筛选标记: | 新霉素Neo/G418 |
| 克隆菌株: | DH5α等大肠杆菌 |
| 培养条件: | 37℃，有氧，LB |
| 表达宿主: | 293T等哺乳细胞 |
| 诱导方式: | 无需诱导 |
| 5'测序引物: | pCDNA3.1-F（CTAGAGAACCCACTGCTTAC） |
| 3'测序引物: | pCDNA3.1-R（TAGAAGGCACAGTCGAGG） |
质粒简介
pCDNA3.1(+)是来自pCDNA3的5.4kb载体，设计用于哺乳动物宿主中的高水平稳定和瞬时表达。大多数哺乳动物细胞可以进行高水平的稳定和非复制性瞬时表达。人类巨细胞病毒立即早期（CMV）启动子，用于在广泛哺乳动物细胞中的高水平表达。前向（+）和反向（-）方向上的多个克隆位点，以促进克隆用于选择稳定细胞系的新霉素抗性基因。在潜伏感染SV40或表达SV40大T抗原（例如COS-1，COS-7）的细胞系中的异常复制。
pcDNA3.1(+) and pcDNA3.1(-) are 5.4 kb vectors derived from pcDNA3 and designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
• Human cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells
• Multiple cloning sites in the forward (+) and reverse (-) orientations to facilitate cloning
• Neomycin resistance gene for selection of stable cell lines
• Episomal replication in cells lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. COS-1, COS-7). The control plasmid, pcDNA3.1/CAT, is included for use as a positive control for transfection and expression in the cell line of choice.
This pcDNA3.1(+)vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Geneticin® selectable marker and a forward-orientation multiple cloning site.
pcDNA3.1(+) and pcDNA3.1(-) are 5.4 kb vectors derived from pcDNA3 and designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
• Human cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells
• Multiple cloning sites in the forward (+) and reverse (-) orientations to facilitate cloning
• Neomycin resistance gene for selection of stable cell lines
• Episomal replication in cells lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. COS-1, COS-7). The control plasmid, pcDNA3.1/CAT, is included for use as a positive control for transfection and expression in the cell line of choice.
This pcDNA3.1(+)vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Geneticin® selectable marker and a forward-orientation multiple cloning site.
质粒图谱
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载体pCDNA3.1(+)
¥800 - 1380
