![载体pGL4.79[hRluc/Neo]](https://img1.dxycdn.com/2017/0807/346/3225684599662788739-14.jpg)
- ¥800 - 1380
- HZbscience
- 中国/美国/德国
- HZ0209
- 2025年07月07日
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- 详细信息
- 技术资料
- 保存条件：
-20℃
- 保质期：
半年
- 英文名：
pGL4.79[hRluc/Neo]
- 库存：
200
- 供应商：
沪震生物
- 规格：
5μl
pGL4.79[hRluc/Neo]
产品信息
| 产品货号 | 产品名称 | 产品规格 | 优惠价 |
|---|---|---|---|
| HZ0209 | pGL4.79[hRluc/Neo] | 20μl |
¥请询价 |
使用说明
沪震质粒平台的各批次质粒菌株发货前均经过严格的多重验证，如存在质量问题，请在收到产品的三个月内通知我司。收到质粒后请短暂离心，取2μl转化至对应感受态中，挑取单克隆重新提取质粒后使用。
基本信息
| 启动子: | SV40 |
|---|---|
| 复制子: | pUC ori，SV40 ori |
| 终止子: | SV40 poly(A) signal |
| 质粒分类: | 哺乳细胞，信号通路报告载体 |
| 质粒大小: | 4879bp |
| 原核抗性: | Amp |
| 筛选标记: | Neo |
| 克隆菌株: | DH5α |
| 培养条件: | 37℃，有氧 LB |
| 表达宿主: | 哺乳细胞 |
| 诱导方式: | 无须诱导，瞬时表达 |
| 5'测序引物: | RVP3:CTAGCAAAATAGGCTGTCCC |
| 3'测序引物: | 根据序列设计引物 |
质粒简介
The pGL4.79[hRluc/Neo] Vector encodes the luciferase reporter gene hRluc (Renilla reniformis) and is designed for high expression and reduced anomalous transcription. This vector also contains a mammalian selectable marker for neomycin resistance in which the number of transcription factor binding sites has been reduced and mammalian codon usage optimized. The pGL4 Vectors are engineered with fewer consensus regulatory sequences than the pGL3 Vectors and a synthetic reporter gene that has been codon optimized for mammalian expression. The pGL4.79[hRluc/Neo] Vector is a basic vector with no promoter. However, it contains a multiple cloning region that allows cloning of a promoter of choice.
![载体pGL4.79[hRluc/Neo]](https://www.miaolingbio.com/uploads/allimg/160904/4-160Z4100G6105.png)
质粒图谱
![载体pGL4.79[hRluc/Neo]](https://www.miaolingbio.com/uploads/160904/4-160Z41005502B.png)
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载体pGL4.79[hRluc/Neo]
¥800 - 1380
