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Features of the TaqMan® Fast Advanced Master Mix include:
• Best-in-class performance—superior sensitivity, accuracy, dynamic range, and specificity compared to standard mixes in standard mode
• Engineered for enhanced benchtop stability—stable at room temperature for up to 72 hours in preassembled reactions
• Optimized for multiplexing—validated for duplexing with exogenous and endogenous internal positive control assays
• Reduced run times on fast and standard instrumentation—optimized for fast mode on fast instruments and fast cycling conditions on standard instruments
• Seamlessly transitions into your experiments—validated with Applied Biosystems® TaqMan® Assays for Gene Expression and microRNAs, and TaqMan® Array Micro Fluidic Cards
Best-in-Class Performance
TaqMan® Fast Advanced Master Mix has been designed to provide performance equal to or better than the results you currently expect from your standard master mix. The master mix has been benchmarked against the leading suppliers' standard and fast master mixes to help ensure that it succeeds in providing best-in-class sensitivity, accuracy, dynamic range, and specificity. The impressive sensitivity of the mix showcased in Figure 1 compares the Ct values of TaqMan® Fast Advanced Master Mix and TaqMan® Universal PCR Master Mix across a panel of gene expression assays.
Benchtop Stability for High-Throughput Handling and Convenience
TaqMan® Fast Advanced Master Mix has been engineered to retain a high level of performance in preassembled reactions for up to 72 hours. The stability of this mix provides users of high-throughput, liquid handling systems the assurance that the results on the first plate will mimic those of the last plate. Figure 2 shows an assay that was run upon assembly (time 0) and after 72 hours of incubation at 30°C, simulating the most extreme room temperature scenario.
For those with less extreme throughput needs, the enhanced stability of this master mix provides an overall added convenience to your workflow, as you are no longer constrained to immediately running your plates upon assembly.
Optimized for Multiplexing
We realize that confidence is paramount when it comes to your results. For added confidence in the results you see in every well, TaqMan® Fast Advanced Master Mix has been designed to help deliver accurate results for duplex reactions using an internal positive control (IPC). Figure 3 shows results for the experimental target β-actin (gene name ACTB), which was serially diluted and amplified in single-target reactions and duplex reactions.
Validated for MicroRNA Assays
TaqMan® Fast Advanced Master Mix has been validated for use with multiple real-time PCR applications, including microRNA assays. The formulation provides high specificity and dynamic range, the two most critical performance attributes that define successful results when working with microRNAs. The data in Figure 4 demonstrate excellent PCR linearity over a 6-log range of input template.
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文献和实验TaqMan Primer and Probe Design
Adapted from TaqMan® One-Step RT-PCR Master Mix Reagents Kit Instruction Design of Probes Keep the G-C content in the 20 to 80% range. Avoid runs of an identical nucleotide. This is especially true for guanine, where runs
这两个条件我们才可以选择快速反应模式。而 Standard Mode 和 Fast Mode 的主要区别在于不同模式下仪器升降温速率不同，快速模式下升降温速度更快。下图列举了当前部分用于基因表达和基因分型的 Master Mix 所支持的反应模式，供大家参考（图 4）。另外针对当前使用较为广泛的体外诊断试剂盒，如新冠检测试剂盒，大家在运行模式选择时需要根据试剂盒说明书做相应的设置，保证实验设置与试剂盒说明书一致。 图 4 常见基因表达和基因分型 master mix 支持反应模式整理
CNV Analysis Using TaqMan Copy Number Assays
genotyping master mix containing AmpliTaq Gold DNA polymerase, ultra‐pure, containing dNTPs (recommended for optimal performance of TaqMan copy number assays) TaqMan copy number assay mix (pre
