pMIB v5-His B产品信息

pMIB v5-His B产品信息

订购信息

货号	载体名称	出品公司	载体用途	价格	到货周期
HZ0031	pMIB v5-His B		昆虫细胞表达载体	1000	现货

产品参数

Mammalian Selection：Blasticidin
Promoter：OplE2
Vector Type：pMIB
Cloning Method：Restriction Enzyme/MCS
Protein Tag or Fusion：V5 Epitope Tag，His Tag (6x His)
Protein Tag Position (to your gene)：C-terminal
测序引物：

Primer	Sequence	pMoles Supplied
OpIE2	Forward 5′-CGCAACGATCTGGTAAACAC-3′	329
OpIE2	Reverse 5′-GACAATACAAACTAAGATTTAGTCAG-3′	250

载体描述

pMIB/V5-His is a 3.6 kb vector designed for constitutive expression and secretion of recombinant proteins from Sf9, Sf21, and High Five? insect cells. Secretion of proteins into serum-free medium simplifies purification, making it easier to harvest protein from cultured cells. pMIB/V5-His has several features to facilitate expression of recombinant proteins in insect cells including:

1. The OpIE2 promoter for constitutive expression
2. The honeybee melittin (HBM) secretion signal to allow secreted expression of your protein
3. The Blasticidin resistance gene for rapid selection of stably transfected cell lines in two weeks
4. C-terminal V5-epitope and polyhistidine tag for detection with Invitrogens Anti-V5 antibody and purification with nickel-chelating resin

载体序列

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