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- bsm-33226M
- 2025年10月16日
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- 规格：
50ul/100ul/200ul/200ug
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 规格： | 200ug | 产品价格： | ￥5600.0 |
| 产品编号 | bsm-33226M |
| 英文名称 | RuBisCO Mouse mAb |
| 中文名称 | 核糖二磷酸羧化酶大链单克隆抗体 |
| 英文别名 | rbcL; RBL_SPIOL; Ribulose bisphosphate carboxylase large chain; RuBisCO-large subunit; ribulose-1, 5-bisphosphate carboxylase/oxygenase large subunit. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Arabidopsis Thaliana |
| 抗体来源 | Mouse |
| 免疫原 | D-Ribulose 1,5-Diphosphate Carboxylase purified |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein G |
| 克隆类型 | Monoclonal |
| 理论分子量 | 52 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cell Biology > Other Antibodies > Plant related targets Metabolism > Pathways and Processes > Metabolic signaling pathways > Energy transfer pathways > Energy Metabolism Signal Transduction > Metabolism > Energy Metabolism |
| 亚基 | Heterohexadecamer of 8 large chains and 8 small chains. |
| 亚细胞定位 | Plastid, chloroplast. |
| 翻译后修饰 | The disulfide bond which can form between Cys-247 in the large chain dimeric partners within the hexadecamer appears to be associated with oxidative stress and protein turnover. The disulfide bonds reported in 1RBO may be the result of oxidation during crystallization. |
| 相似性 | Belongs to the RuBisCO large chain family. Type I subfamily. |
| 功能 | RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process (PubMed:2928307) (Probable). Both reactions occur simultaneously and in competition at the same active site (Probable). Binds to abscisic acid (ABA) which has weakly inhibits carboxylation and more strongly inhibits enzyme activation (PubMed:26197050). |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
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文献和实验[IF={{ 8.2 }}] {Wen Wang. et al. Characterization of a novel γ-type carbonic anhydrase, Sjγ-CA2, in Saccharina japonica: Insights into carbon concentration mechanism in macroalgae. INT J BIOL MACROMOL. 2024 Apr;263:130506} {WB} {Saccharina japonica}
[IF={{ 4.9 }}] {Qilei Zhang. et al. Adaptation of the Invasive Plant Sphagneticola trilobata to Flooding Stress by Hybridization with Native Relatives. INT J MOL SCI. 2024 Jan;25(12):6738} {WB} {Sphagneticola trilobata}
T-Cell Activation Using mAb to CD3
One of the most common ways to assess T cell activation is to measure T cell proliferation upon in vitro stimulation of T cells via antigen or agonistic antibodies to TCR. This protocol is written as a starting point for examining in vitro proliferation of mouse
WHOLE MOUNT IN SITU HYBRIDIZATION. Mouse Embryos
. Use gloves and aerosol barrier tips. Linearize the plasmid and check the digest. Phenol extract. Extract twice with chloroform:isoamyl alcohol (24:1) Ethanol precipitate ( 1/2 vol 7.5 M NH4 OAc + 2.5 vol 100
Hybrid Resistance by Mouse NK Cells In Vitro
A purified NK cells to kill H2d target cells can be reversed by adding MAb reagents that bind to either Ly49A or H2-Dd . We and others have extended these early findings to the Ly49C and I subsets of NK cells, and have recently detected positive signaling
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