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- 2025年10月24日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-19227R |
| 英文名称 | NFS1 Rabbit pAb |
| 中文名称 | 半胱氨酸脱硫酶NFS1抗体 |
| 英文别名 | Cysteine desulfurase; Cysteine desulfurase, mitochondrial; HUSSY 08; IscS; mitochondrial; NFS1; NFS1 nitrogen fixation 1; NFS1 nitrogen fixation 1 homolog(S. cerevisiae); NFS1_HUMAN; NIFS; nitrogen fixation 1(S. cerevisiae, homolog); nitrogen fixation gene 1; nitrogen-fixing bacteria S, homolog of; nitrogen-fixing bacteria S-like protein; OTTHUMP00000030799. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, ELISA=1:5000-10000 Not yet tested in other applications. |
| 交叉反应 | (Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Saccharomyces cerevisiae) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human NFS1 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 50 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Metabolism > Pathways and Processes > Cofactors, Vitamins / minerals > Vitamins / minerals Metabolism > Pathways and Processes > Metabolic signaling pathways > Amino acid metabolism Signal Transduction > Metabolism > Amino Acids Signal Transduction > Metabolism > Vitamins / Minerals |
| 亚细胞定位 | Cytoplasm. Nucleus and Mitochondrion. |
| 组织特异性 | Predominantly expressed in heart and skeletal muscle. Also found in brain, liver and pancreas. |
| 相似性 | Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily. |
| 功能 | Catalyzes the removal of elemental sulfur from cysteine to produce alanine. It supplies the inorganic sulfur for iron-sulfur (Fe-S) clusters. May be involved in the biosynthesis of molybdenum cofactor. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Iron-sulfur clusters are required for the function of many cellular enzymes. The proteins encoded by this gene supply inorganic sulfur to these clusters by removing the sulfur from cysteine, creating alanine in the process. This gene uses alternate in-frame translation initiation sites to generate mitochondrial forms and cytoplasmic/nuclear forms. Selection of the alternative initiation sites is determined by the cytosolic pH. The encoded proteins belong to the class-V family of pyridoxal phosphate-dependent aminotransferases. Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2010] |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {ICC/IF} | {1:100-500} |
| {IF} | {1:100-500} |
| {ELISA} | {1:5000-10000} |
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文献和实验[IF={{ 2.9 }}] {Jiang You. et al. NFS1 inhibits ferroptosis in gastric cancer by regulating the STAT3 pathway. J BIOENERG BIOMEMBR. 2024 Sep;:1-15} {WB} {Human,Mouse}
= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
GE Healthcare Benzamidine Sepharose™ 6B is p-aminobenzamidine covalently attached to Sepharose 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is a synthetic inhibitor of trypsin-like serine protease. Trypsin and trypsin
Nucleic Acid Programmable Protein Arrays: Versatile Tools for Array‐Based Functional Protein Studies
.g., Sigma) 50 mg/ml bis‐sulfosuccinimdylsuberate (BS3 ;Pierce, cat. no. PI 21580) in dimethylsulfoxide (DMSO) 5 mg/ml
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