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文献和实验by the neck, using a sterile metal hook or a bent glass rod, and place the embryo in a 100mm petri dish containing phosphate buffered saline (PBS). Wash several times with PBS by transferring the embryo to fresh petri plates. After removal of all yolk
ChIP protocol for X. laevis Lens1/FoxE3 enhancer
1.5 ml 1.2 mM EDTA 0.5M stock 120 µl 1.1% Triton X-100 10% stock 5.5 ml 0.01% SDS 10% stock 50 µl
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
. Centrifuge the Gelvatol solution at 12,000 rpm for 15 min. in 30 ml Corex tubes in Beckman J2-21 centrifuge at room temperature to remove undissolved particles. Pipette the supernatant into small screw cap bottles. Check pH of Gelvatol solution
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