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文献和实验ChIP protocol for X. laevis Lens1/FoxE3 enhancer
SDS lysis buffer/1x proteinase inhibitor cocktail (2.5 µl of 200 x stock from Upstate EZ ChIP kit). Roughly dissociate heads by pipetting, and transfer them into a 2 ml dounce homogenizer. Total volume is ~700 µl. �SDS lysis
Human Complement Components C4A and C4B Genetic Diversities: Complex Genotypes and Phenotypes
PCR x enhancer solution 50 mM MgSO 4 5 U/µl Platinum Taq DNA polymerase
found in insoluble fractions are to be analyzed using MuDPIT, begin with the steps outlined in Digestion of Insoluble Protein Fractions for MuDPIT analysis . Chromatography solvent A Urea Equipment C18 reversed-phase packing material (5 µm) (e.g
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