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上海希言科学仪器有限公司
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文献和实验Native Chromatin Preparation and Illumina/Solexa Library Construction
(1X PBS containing 0.5 % bovine serum albumin, filtered) T4 DNA ligase (400 U/µL) and 10X buffer Taq polymerase (New England Biolabs) TaqMan PCR master mix (Applied Biosystems) (see Step 38) TE (1X, pH 7.4) Triton X-100
Yeast boiling DNA miniprep (Robzyk & Kassir, Nucl. Acid. Res. 20: 3790, 1992) Harvest 1.5 ml of an ON yeast culture grown under selective conditions (5K, 5 minutes). Resuspend in 100 µl STET (8% sucrose, 50 mM Tris pH 8, 50 mM EDTA, 5% Triton X
(Cibacron Blue F3G-A coupledto Sepharose), ~100 mL packed into an empty column with100–200 mL of bed volume. 3. Binding Buffer: 1% (w/v) CHAPS, 150 mM KCl, and 20 mMTris-HCl, pH 7.5, sterile filtered. 4. Elution Buffer: 1% (w/v) CHAPS, 1.5 M KCl
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