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文献和实验The ribonuclease protection assay (RPA)
P]UTP 1 µl T7 RNA polymerase (Keep at -20°C until use, return to -20°C immediately). Mix by gentle pipetting or flicking and quick spin in a microfuge. Incubate at 37°C for 1 hour. 2. Terminate the reaction by adding 2 µl of DNase. Mix
polymerase (Keep at -20℃ until use, return to -20℃ immediately). Mix by gentle pipetting or flicking and quick spin in a microfuge. Incubate at 37℃ for 1 hour. 2.Terminate the reaction by adding 2 µl of DNase. Mix by gentle flicking and quick spin
Overgo Probing of High-Density Filters
and not trap air bubbles. Each filter is added separately at this time to ensure that each is prehyb'd adequately. Routinely, 7 filters are placed in the same bottle. Make sure that all filters are rolled in the same direction and that the bottle is set
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