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上海希言科学仪器有限公司
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文献和实验Translation Using a Wheat-Germ Extract
Wheat-germ extract provides a reliable, convenient, and easy-to-use system to initiate translation from a wide variety of viral, prokaryotic, and eukaryotic mRNAs and produce full-length polypeptide products (1 ). Translation reactions in vitro
In Vivo and In Vitro Analysis of Poly(A) Length Effects on mRNA Translation
at steady-state level can be directly measured with Western blot or using an easy-to-detect reporter such as luciferase. However, these methods do not measure the association of mRNA with ribosomes, which is more meaningful in understanding the mechanism
1) For easy collection of perfusate samples for analysis, (e.g. nitric oxide synthesis), first pause the pump. 2) Close the stopcock located closest to the pulse dampener. Remove its protective cap and insert a small size syringe, e
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