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- bs-0296G
- 2025年10月10日
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- 规格：
1mg/10mg/50mg
| 规格： | 1mg | 产品价格： | ￥980.0 |
|---|---|---|---|
| 规格： | 10mg | 产品价格： | ￥4800.0 |
| 规格： | 50mg | 产品价格： | ￥12800.0 |
| 产品编号 | bs-0296G |
| 英文名称 | Goat Anti-Mouse IgG H&L |
| 中文名称 | 羊抗小鼠IgG H&L |
| 英文别名 | Mouse Immunoglobulin G; Mo IgG; |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:200-1000, IF Not yet tested in other applications. |
| 交叉反应 | Mouse, Rat, Human |
| 抗体来源 | Goat |
| 免疫原 | Native Mouse IgG |
| 亚型 | IgG |
| 性状 | Lyophilized |
| 纯化方法 | affinity purified by Protein G |
| 克隆类型 | Polyclonal |
| 储存液 | 0.01M PBS (pH7.4). |
| 保存条件 | Store at -20℃ stable for 2 years (lyophilized powder). Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:200-1000} |
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文献和实验E L I S A w i t h p l a t e l e t s
for 1 hour with a 1 :1000 dilution of rat IgG2 a anti-mouse kappa chain monoclonal antibody conjugated to horse-radish peroxidase (LO-MK1 , LO/IMEX) reveal with o-phenylenediamine dihydrochloride (OPD) in revelation solution. SOLUTIONS
Anti-GFP/BrdU In Situ Immunostaining
IgG (Jackson ImmunoResearch, 111-096-006) Biotinylated anti-BrdU (AbCam, ab2284-125) Streptavidin-Alexa Flour 555 (Invitrogen/Molecular Probes, S21381) Goat serum Horse serum BSA 2N HCl DAPI Fluorescent mounting medium (Vectashield
and wash the blot 3 times, 5-7 minutes each, with 0.5% BSA in TBST.11. Incubate with Goat anti-Rabbit/alkaline phosphatase (from Promega; 1:7500 inTBST with 5% BSA) for 1 hour at room temp with shaking. I have also used successfully Amersham ECL detection
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