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- 文献和实验
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1ml
| 产品编号 | bs-0295Ds |
| 英文名称 | Donkey Anti-Rabbit IgG H&L whole serum |
| 中文名称 | 驴抗兔IgG H&L抗血清 |
| 英文别名 | Rabbit Immunoglobulin G; Rb IgG; |
| 交叉反应 | Rabbit |
| 抗体来源 | Donkey |
| 免疫原 | Native Rabbit IgG |
| 亚型 | IgG |
| 性状 | Lyophilized |
| 纯化方法 | Unpurified |
| 克隆类型 | Polyclonal |
| 保存条件 | Store at -20℃ stable for 2 years (lyophilized powder). Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. |
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文献和实验E L I S A w i t h p l a t e l e t s
for 1 hour with a 1 :1000 dilution of rat IgG2 a anti-mouse kappa chain monoclonal antibody conjugated to horse-radish peroxidase (LO-MK1 , LO/IMEX) reveal with o-phenylenediamine dihydrochloride (OPD) in revelation solution. SOLUTIONS
Making Boiled Donkey (or whatever) Serum (BDS) for Blocking
aliquot, but maybe if it's a fresh aliquot use it at 1:400) for 30 minutes at 37oC. 11) Rinse 3 times for 5 minutes each rinse in PHEM-T. 12) Put on secondary (I use Donkey anti-mouse-Rhodamine Red X) at 1:100 in block for 30 minutes at 37oC. 13
ELISA Procedure for Measuring Serum Antibody Titer
Phosphatase Reagents for Alkaline Phosphatase DetectionGoat anti-species IgG whole molecule/alkaline phosphatase conjugate that is appropriate for the source of your antiserum. For example, if the antibodies were raised in a rabbit, use goat anti-rabbit IgG
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