Sample:
HepG2 Cell (Human) Lysate at 30 ug
Hela Cell(Human)Lysate at 30 ug
Primary: Anti- GERP (bs-9432R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 61kD
Observed band size: 61kD
- ￥1180 - 2800
- Bioss
- bs-9432R
- 2025年10月16日
- 产品信息以Bioss网站为准
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-9432R |
| 英文名称 | GERP Rabbit pAb |
| 中文名称 | 环指蛋白27抗体 |
| 英文别名 | RNF27; Lioblastoma expressed ring finger protein; Ring finger protein 27; RNF27; RP11-47A8.4; TRIM8; Tripartite motif containing 8; Tripartite motif protein TRIM8; TRIM8_HUMAN. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat, Chicken, Dog, Horse, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human GERP/TRIM8/RNF27 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 61 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cell Biology > Proteolysis / Ubiquitin > Proteasome / Ubiquitin > Ubiquitin E3 Enzymes > RING Finger E3 Ligase Epigenetics and Nuclear Signaling > Transcription > Cancer susceptibility > Tumor Suppressors Epigenetics and Nuclear Signaling > Transcription > Domain Families > Developmental Families |
| 亚基 | Homodimer. Interacts with SOCS1 (via) SH2 domain and SOCS box. |
| 组织特异性 | Widely expressed. |
| 相似性 | Belongs to the TRIM/RBCC family. Contains 2 B box-type zinc fingers. Contains 1 RING-type zinc finger. |
| 功能 | Probable E3 ubiquitin-protein ligase which may promote proteasomal degradation of SOCS1. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The tripartite motif (TRIM) family of proteins are characterized by a conserved TRIM domain that includes a coiled-coil region, a B-box type zinc finger, one RING finger and three zinc-binding domains. TRIM8 (tripartite motif containing 8), also known as GERP (glioblastoma-expressed RING finger protein) or RNF27 (RING finger protein 27), is a 551 amino acid protein that is thought to function as an E3 ubiquitin-protein ligase that promotes SOCS-1 proteasomal degradation. As a widely expressed homodimer, TRIM8 localizes to nuclear bodies and contains two B box-type zinc fingers and one RING-type zinc finger. TRIM8 is expressed in lung, heart, brain and skeletal muscle, with low levels detected in intestine, placenta, leukocytes and liver. The gene encoding TRIM8 maps to human chromosome 10q24.32. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
Sample:
Placenta (Mouse) Lysate at 40 ug
Primary: Anti- GERP (bs-9432R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 61kD
Observed band size: 61kD
Placenta (Mouse) Lysate at 40 ug
Primary: Anti- GERP (bs-9432R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 61kD
Observed band size: 61kD
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文献和实验相关实验
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GERP Rabbit pAb(bs-9432R)-50ul/100ul/200ul
￥1180 - 2800
