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- bsm-41680M
- 2025年10月24日
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100ug/200ug
| 规格： | 100ug | 产品价格： | ￥2500.0 |
|---|---|---|---|
| 规格： | 200ug | 产品价格： | ￥5600.0 |
| 产品编号 | bsm-41680M |
| 英文名称 | Human S100B Mouse mAb |
| 中文名称 | 人S100B蛋白单克隆抗体 |
| 英文别名 | NEF; Protein S100 B; Protein S100-B; S 100 calcium binding protein beta chain; S 100 protein beta chain; S 100 protein beta subunit; S-100 protein beta chain; S-100 protein subunit beta; S100; S100 calcium binding protein B; S100 calcium binding protein beta(neural); S100 calcium binding protein beta chain; S100 calcium-binding protein B; S100 protein beta chain; S100B_HUMAN; S100beta; S100 β; S100-β; S100β. |
| 产品应用 | WB=1:500-2000, ELISA=1:5000-10000 Not yet tested in other applications. |
| 交叉反应 | Human |
| 抗体来源 | Mouse |
| 免疫原 | Recombinant human S100B protein |
| 亚型 | IgG1 |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Monoclonal |
| 理论分子量 | 10 kDa |
| 储存液 | 0.01M PBS (pH7.4). |
| 研究领域 | Cell Biology > Cell Cycle > Cell differentiation Epigenetics and Nuclear Signaling > Cell cycle > Cell Differentiation Signal Transduction > Antibodies > s100 beta Stem Cells > Neural Stem Cells > Glial Restricted Lineage > Astrocyte |
| 亚基 | Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain. The S100B dimer binds two molecules of STK38 (By similarity). The S100B dimer interacts with two molecules of CAPZA1. |
| 亚细胞定位 | Cytoplasm. Nucleus. |
| 组织特异性 | Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues. |
| 相似性 | Belongs to the S-101 family. Contains 2 EF-hand domains. |
| 功能 | Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | S100 beta is a member of the S100 family of proteins containing 2 EF-hand calcium binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {ELISA} | {1:5000-10000} |
Lane 1: Recombinant human S100B protein, N-His
Primary: Anti-Human S100B (bsm-41680M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 10 kDa
Observed band size: 10 kDa
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